作　　者： (马雪曼）; (于明薇）; (张甘霖）; (于洁）; (曹可心）; (孙旭）; (杨国旺）; (王笑民）;

机构地区： 北京中医药大学,北京100029 首都医科大学附属北京中医医院,北京100010

出　　处： 《中国实验动物学报》 2017年第4期386-390,共5页

摘　　要： 目的探究不同部位肺癌皮下移植瘤存在的差异,为肺癌研究者提供提供基础依据。方法将稳定表达虫萤光素酶的小鼠Lewis肺腺癌细胞(ll2-luc-m38)分别注射于C57 BL/6小鼠的右腋皮下、右腹股沟皮下、脚垫皮下,定期利用小动物活体成像系统观察小鼠皮下移植瘤成瘤情况及转移情况,观察不同部位荷瘤小鼠的生存时间和死亡率,肿瘤组织取材,采用石蜡包埋、切片、HE染色做出病理学诊断。并将小鼠处死后立即进行肺部组织固定、观察转移灶。对皮下移植瘤行切除术观察小鼠术后生存情况和转移情况。结果腋下组和腹股沟组成瘤时间较早,成瘤率为100%,脚垫组成瘤时间较晚,成瘤率为33%;腹股沟组和腋下组瘤体积增长迅速,其中腹股沟组瘤体积增长最快;接种后第21天腋下组70%的小鼠出现了肺转移,转移灶数目较多;腹股沟组50%小鼠出现肺转移,且转移灶数目较少;脚垫组小鼠未观察到肺转移灶。脚垫组小鼠死亡率最高。腹股沟组和腋下组小鼠可行皮下移植瘤切除术,术后存活率为100%。结论小鼠lewis肺癌皮下移植瘤模型中,腹股沟组和腋下组成瘤率高,可耐受移植瘤切除术,手术死亡风险低,便于监测,操作简单且可重复性高,其中腋下组转移性更好。 Objective Subcutaneous transplantation Lewis lung carcinoma model is commonly used in experimental studies. Researchers often choose different transplantation sites to create the models while little attention was paid on the effect of different inoculation sites on the formation of transplanted tumors. The aim of this study was to compare the effect of tumor cell inoculation at different sites on tumor formation in mice. Methods Lewis lung adenocarcinoma（ ll2-lucm38） cells stably expressing luciferase protein were subcutaneously injected into C57 BL/6 mice at the right armpit,right groin,or footpad,respectively. An IVIS spectrum in vivo imaging system was used to observe the tumor and metastasis formation. The survival time and mortality were recorded. H-E stained pathology was performed to examine the histological changes of the lung tissues and tumor metastesis. Results The tumor formation time was earlier in the armpit and groin groups,both with a tumor formation rate of 100%,while the tumors occurred later,with a tumor formation rate of 33% in the footpad group. The pulmonary metastasis rate was 70% in the groin group,50% in the ampit group,and 0% in the footpad group,at the 21 st day after inoculation. The footpad group had a high mortality. The tumors in the groin group andarmpit group can be surgically resected,with a postoperative survival rate of 100%. Conclusions In this mouse model of subcutaneously transplanted Lewis adenocarcinoma,the groin and ampit groups have advantages such as a high tumor formation rate,good tolerance of tumor resection,low surgical mortality rate,easy to monitor,simple operation and high reproducibility. The axillary group has an even higher metastasis rate.

关 键 词： 肺癌 皮下移植瘤 动物模型