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4株天牛致病菌对川硬皮肿腿蜂的毒力测定
Virulence Determination of Four Monochamus alternatus Pathogens on Scleroderma sichuanensis

作  者: (陶园媛); (向茂榕); (刘昊泽); (王理顺); (何勇); (杨伟); (杨桦); (杨春平);

机构地区: 四川农业大学林学院森林保护实验室,四川成都611130

出  处: 《四川林业科技》 2017年第4期30-33,共4页

摘  要: 利用肿腿蜂携菌防治天牛,可发挥肿腿蜂主动搜索和病菌致病力强的优势,收到较单纯放蜂或喷施菌剂更好的效果。但需保证携带的病菌不会显著影响肿腿蜂的寄主搜索能力。为了利用川硬皮肿腿蜂携带病原菌防治松墨天牛,本文测定了4株松墨天牛致病菌的孢子粉和孢子悬浮剂对川硬皮肿腿蜂的毒力。结果表明:雌成蜂携带粉剂孢子的死亡率高于携带悬浮剂孢子的死亡率;当喷施孢子悬浮剂时,4个菌株的累计死亡率随浓度的增加而增加;白僵菌对川硬皮肿腿蜂的毒力较绿僵菌高,致死速率也较绿僵菌大。研究结果可为川硬皮肿腿蜂携菌防治松墨天牛的菌种选择和剂型研发提供依据。 Making use of Bethylidae carrying pathogens to prevent and control the longicorns could play advantage of active search of Bethylidae and strong pathogenicity of pathogens, better than releasing Bethylidae only or spraying microbial inoculum. However, it was necessary to ensure that the carrying pathogens would not significantly affect the Bethylidae' host search ability. In order to use Scleroderma sichuanens/s carrying pathogens to prevent and control Monochamus alternatus, this paper measured the virulence of the conidial powder and spore suspensions of four strains of pathogens on S. sichuanensis. The results showed that the mortality rate of the powder spores in S. sichuanensis was higher than that of the suspension spores. When spraying spore suspension, the cumulative mortality of four strains increased along with the increasing concentration. For S. sichuanensis, the virulence of Beauveria bassiana was relatively higher than that of Metarhizium anisopliae, and the lethal rate of B. bassiana was also higher than that of M. anisopliae. Research results could provide some basis data for the selection of strains and development of microbial inoculum which was useful for S. sichuanensis carrying pathogens to prevent the M. alternatus.

关 键 词: 川硬皮肿腿蜂 松墨天牛 白僵菌 绿僵菌 毒力测定

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