作　　者： (罗碧）; (冯洁深）; (白和灵）; (刘超）; (赵锦龙）; (谭亚玲）; (徐津）;

机构地区： 云南农业大学农学与生物技术学院,云南昆明650201 云南农业大学稻作研究所,云南昆明650201

出　　处： 《云南农业大学学报（自然科学版）》 2017年第4期571-576,共6页

摘　　要： 【目的】对野生稻中木糖基转移酶基因开展初步研究,为进一步探索单子叶植物中木葡聚糖的合成及功能研究打下坚实的基础。【方法】利用同源克隆的方法,从普通野生稻(Oryza rufipogon)中克隆了1个糖基转移酶基因OrGT1。【结果】序列分析结果显示:该基因具有1个保守的Glyco-transf-34结构域,属于糖基转移酶34家族的基因。系统进化分析表明:该基因可能是1个木葡聚糖合成中的木糖转移酶基因。实时荧光定量分析表明:该基因在普通野生稻的在根、茎、叶和穗中均有表达,但在不同组织中表达有明显差异,其中茎的表达量最高,而根中的表达量最低。【结论】OrGT1基因在进化过程中高度的功能保守性,推测OrGT1(t)基因可能是1个参与木葡聚糖合成的木糖基转移酶基因,该研究结果为进一步探索该基因的功能打下良好的基础。 [ Purpose ] In this study, the preliminary study on the in wild rice was carried out, which laid a solid foundation for the and function of xyloglucan in monocotyledonous plants. [ Method ] glycosyltransferase gene OrGT1 （t） further exploration of the synthesis A glycosyltransferases zene OrGT1（t） was cloned from Oryza rufupogon using homologous cloning methods. [Results] Sequence analy- sis revealed that the gene has a conservative Glyco-transf-34 domain, belonging to the Glyco-transf-34 gene falimy. Phylogenetic analysis showed that OrGT1 may be a xyloglucan xylosetransferase genein- volve in xyloglucan synthesis. RT-qPCR analysis indicated that the OrGT1 expressed in all checkedorgans, including the root, stem, leaf and spike was different among the organs, and there were the est in the root. [ Conclusion] The OrGT1 gene is lutionary process, suggesting that the OrGTI（t） can synthesis, the results lay a good foundation in 0. rufupogon. But the transcriptional quantity highest expression quantity in the stem and the Iow- a high degree of functional conservation in the evo- gene may be a xyloglucanase gene involved in xyloglu- for further exploring the function of the OrGT1 gene.

关 键 词： 普通野生稻 糖基转移酶基因 基因克隆 实时荧光定量