作　　者： (葛玉琰）; (孙奕）; (张鹏）; (陈士林）; (友田健久）; (江蔚新）;

机构地区： 中国中医科学院中药研究所,北京100700 哈尔滨商业大学,黑龙江哈尔滨150076

出　　处： 《沈阳药科大学学报》 2017年第8期674-679,共6页

摘　　要： 目的基于DNA条形码分子鉴定技术鉴定升麻药材真伪,并制备升麻饮片标准汤剂,建立质量标准。方法对不同产地药材进行DNA条形码基原鉴定;根据中药饮片标准汤剂制备原则,将鉴定为升麻的饮片制备标准汤剂,分析并计算异阿魏酸的转移率和升麻饮片标准汤剂的出膏率,建立升麻饮片标准汤剂的质量标准。结果 10批药材经分子鉴定均为兴安升麻(Cinicifuga dahurica(Turcz)Maxim)。在作者所建立的制备条件下,异阿魏酸转移率为42.75%~65.50%,出膏率为12%~26%,10批升麻饮片标准汤剂指纹图谱相似度大于90.2%。结论利用分子鉴定与标准汤剂的质量分析相结合的方法评价升麻的药材质量,同时建立了规范的升麻标准汤剂制备方法及其质量评价体系。 Objective To prepare the standard decoction of Cimicifuga radix slices and establish its quality standard based on DNA barcoding technique. Methods Total genomic DNA was extracted of Cimicifuga Radix from different places of product. According to the standard of Chinese herbal medicine decoction preparation principle, the standard decoction of Cimicifuga radix slices was prepared to calculate the transfer rate of 3-Hydroxy-4-methoxycinnamic acid as well as the cream rate of standard decoction of Cimicifuga radix slices. In addition, the quality standard of decoction of Cimicifuga radix slices was also established based on the standard of Chinese herbal medicine decoction preparation principle. Results Ten samples were identified as Cimicifuga dahurica（ Turcz. ）Maxim.. 3-Hydroxy-4-methoxycinnamic acid transfer rate ranged from 42.75% to 65.50% and cream rate was at the range of 12 % -26%. The fingerprint similarity of 10 batches of Cimicifuga radix slices standard decoction was 〉 90%. Conclusions It is a simple and reliable method combing molecular identification with HPLC analysis to evaluate the quality of Cimicifuga radix. The established preparation method can be used to establish the standard decoction of Cimicifuga radix slices.

关 键 词： 升麻 分子鉴定 饮片标准汤剂 质量标准 异阿魏酸