作　　者： (康悦）; (王灿灿）; (王淼）; (赵春杰）;

机构地区： 沈阳药科大学药学院,辽宁沈阳110016 沈阳熙正贸易有限公司,辽宁沈阳110021

出　　处： 《沈阳药科大学学报》 2017年第8期640-643,666,共5页

摘　　要： 目的建立一种同时测定皮炎散复方制剂中阿魏酸、毛蕊花糖苷和哈巴俄苷含量的方法。方法采用HPLC法,色谱柱:Xpurisil C_(18)柱(250 mm×4.6 mm,5μm);流动相:乙腈-体积分数为0.5%的甲酸溶液;流速:1.0 m L·min^(-1);检测波长:0~30 min,波长为330 nm,30~50 min,波长为278 nm;进样量:15μL;柱温:35℃。结果阿魏酸、毛蕊花糖苷和哈巴俄苷质量浓度分别在4.0~24.0、24.0~144.0和4.0~24.0 mg·L^(-1)内与峰面积呈良好的线性关系;阿魏酸、毛蕊花糖苷和哈巴俄苷的平均回收率分别为98.9%、99.0%和97.0%,RSD分别为1.8%、1.2%和1.1%(n=6)。结论本方法可用于皮炎散复方制剂中阿魏酸、毛蕊花糖苷和哈巴俄苷含量测定。 Objective To establish a method for determining the contents of ferulic acid, acteoside and harpagoside in Piyan San. Methods The separation was performed on a XPurisil-C18 column （250 mm x 4.6 mm,5 Ixm）with acetonitrile-water（ containing 0. 5% （~o）formic acid）as the mobile phase by gradient elution at the flow rate of 1.0 mL. min - 1. The detection wavelength was 300 nm for 0 - 30 rain ,278 nm for 30 -50 min. And the column temperature was 35 ^（2. Results The results show that ferulic acid, Mullein glucoside, Haba Russian glycoside concentration respectively in 4.0 - 24.0 mg. L - 1,24.0 - 144.0 mg. L - and 4.0 -24.0 mg. L-1 with peak area had a good linear relationship. The average recovery rate was 98.9% ,99.0% and 97.0%, with RSD of 1.8%, 1.2% and 1.1% （n = 6 ）. Conclusions The established method for determining the content of ferulic acid, acteoside and harpagoside in Piyan San is Quick, simple and accurate.

关 键 词： 皮炎散 阿魏酸 毛蕊花糖苷 哈巴俄苷 高效液相色谱法 含量测定