作　　者： (秦阳）; (高颖）; (高英）; (李卫民）;

机构地区： 广州中医药大学中药学院,广州510006

出　　处： 《中国实验方剂学杂志》 2017年第17期144-148,共5页

摘　　要： 目的:初步探索桑白皮黄酮提取物对2型糖尿病大鼠非酒精性脂肪肝的治疗作用及机制研究。方法:将24只雄性大鼠随机分为3组,分别为正常组、模型组、桑白皮黄酮组(1.0 g·kg^(-1))。采用腹腔注射小剂量链脲佐菌素(streptozocin,STZ)结合高脂饲料喂养的方法,建立2型糖尿病伴非酒精性脂肪肝的模型,灌胃治疗16周时,观察比较桑白皮黄酮提取物对各组大鼠血清中空腹血糖(FBG),总胆固醇(TC),甘油三酯(TG),低密度脂蛋白胆固醇(LDL-C),糖耐量(OGTT)变化,油红O染色和苏木素-伊红(HE)染色观察肝脏病理改变,实时荧光定量聚合酶链式反应(Real-time PCR)检测肝脏血管内皮生长因子(vascular endothelial growth factor,VEGF),血小板衍生因子(platelet derived growth factor,PDGF)mRNA的表达。结果:灌胃干预16周后,与正常组比较,模型组大鼠FBG,TC,TG,LDL-C,含量均显著高于正常组(P<0.05),应用桑白皮黄酮治疗后,上述生化指标较模型组均有显著下降(P<0.05)。糖耐量试验显示模型组胰岛素敏感性下降,应用桑白皮黄酮治疗后胰岛素敏感性较模型组有显著改善。油红O染色结果显示模型组肝细胞胞浆内可见大量红染的脂肪滴,而桑白皮黄酮组肝细胞胞浆内未见红染的脂肪滴。HE染色结果显示模型组肝小叶中肝细胞发生明显的小泡样脂肪变性,部分肝细胞中脂滴融合成大的脂肪空泡,小叶中可见点状坏死,周围有炎性细胞浸润,而桑白皮黄酮组肝细胞结构正常。模型组肝脏中VEGF,PDGF mRNA表达量较正常组有显著升高,应用桑白皮黄酮治疗后,肝脏中VEGF,PDGF mRNA表达量明显降低(P<0.05)。结论:桑白皮黄酮提取物对实验性2型糖尿病大鼠非酒精性脂肪性肝有一定的防治作用,其作用机制可能与抑制肝脏中VEGF,PDGF mRNA的表达有关。 Objective ： To explore the therapeutic effect and mechanism of Cortex Mori flavone extracts on nonalcoholic fatty liver in type 2 diabetic rats. Method： Total 24 male rats were randomly divided into three groups ： normal group, model group and Cortex Mori flavone extracts group （ 1.0 g · kg^-1 ）. The model of type 2 diabetes mellitus with nonalcoholic fatty liver was established by intraperitoneal injection of low dose streptozocin （STZ） combined with high fat diet. At the 16th week of gavage treatment, the fasting blood glucose （FBG）, total cholesterol （TC） , triglyceride （TG） , low density lipoprotein cholesterol （LDL-C） , and oral glucose tolerance test （OGTT） were observed and compared. Oil red O staining and HE staining were used to observe the pathological changes of the liver, and Real-time PCR was used to detect the expression of vascular endothelial growth factor （VEGF） and platelet derived growth factor （PDGF） mRNA. Result： After 16 weeks of intervention, the levels of fasting blood glucose （FBG） , total cholesterol （TC） , triglyceride （TG）, and low density lipoprotein cholesterol （LDL-C） in model group were significantly higher than those in control group （P 〈 0.05 ）. After the application of Cortex Mori flavone extracts, the above biochemical indexes dropped significantly in Cortex Mori flavone extracts group. As compared with normal control group, the oral glucose tolerance test showed that the insulin sensitivity was decreased in the model group, and it was significantly improved after the application of Cortex Mori flavone extracts （P 〈 O. 05）. The results of oil red O staining showed that a large amount of red dye was observed in the cytoplasm of hepatocytes in the model group, but no red dye was found in the cytoplasm of the hepatocytes of the Cortex Mori flavone extracts group. HE staining showed that the liver cells in the model group had obvious vesicular steatosis, and the lipid droplets in the partial hepatocytes were f

关 键 词： 桑白皮黄酮 型糖尿病 非酒精性脂肪肝 血管内皮生长因子 血小板衍生因子