作　　者： (李小宝）; (余章昕）; (邵泰明）; (陈光英）; (宋小平）; (韩长日）;

机构地区： 海南师范大学热带药用植物化学教育部重点实验室,化学与化工学院,海口571158

出　　处： 《中国实验方剂学杂志》 2017年第17期58-62,共5页

摘　　要： 目的:研究沙煲暗罗精油的主要化学成分,初步研究其抗肿瘤活性,为进一步研究开发沙煲暗罗提供实验依据。方法:采用水蒸气蒸馏法从沙煲暗罗鲜叶中提取精油,计算收率,利用气相色谱-质谱(GC-MS)对其化学成分进行分析。MTT(四甲基偶氮唑盐)法检测精油对白血病细胞(K-562),肝癌细胞(BEL-7402),肺癌细胞(SPC-A-1)和胃癌细胞(SGC-7901)增殖抑制作用。结果:水蒸气蒸馏法提取沙煲暗罗叶精油收率为0.83%,分离出65个峰,鉴定出44种化学成分,占精油总量的84.382%。其中主要成分为邻苯二甲酸单(2-乙基己基)酯(16.824%),二苯胺(10.321%),邻苯二甲酸二异丁酯(8.904%)和桉油烯醇(4.920%)。结论:抗肿瘤活性结果表明沙煲暗罗叶精油对4种肿瘤细胞株显示较好的抑制活性,在高剂量组中对K-562,BEL-7402,GSC-7901的细胞增殖抑制率均可以达到80%以上。其中对白血病细胞K-562和肝癌细胞BEL-7402均表现出很好的抑制活性,其半数抑制浓度(IC_(50))分别为3.78,8.14 g·L^(-1),且2种活性都强于阳性对照药丝裂霉素C。精油对胃癌细胞SGC-7901也表现一定的抑制活性,其IC50为22.94 g·L^(-1)。 Objective： To study the main chemical components and anti-tumor activities of essential oil from the leaves of Polyalthia consanguinea, and provide experimental basis for further research and development of P. consanguinea. Method： The essential oil from the leaves of P. consanguinea was extracted by steam distillation method to calculate its yield; then gas chromatography-mass spectrometry （GC-MS） was used to analyze its chemical components. MTT assay was used to investigate the inhibitory effect of essential oil on the proliferation of leukemia cells （K-562） , liver cancer cells （ BEL-7402）, lung cancer cells （ SPC-A-1 ） and gastric cancer cells （SGC-7901）. Result： The extraction yield of P. consanguinea oil was 0. 83% ; 65 chromatographic peaks were detected and 44 compounds were identified, accounting for 84. 382% of the total essential oil. The major components were monoethylhexyl phthalate （ 16. 824% ）, diphenylamine （ 10. 321% ）, diisobutyl phthalate （8. 904% ） and spathulenol （4. 920% ）. Conclusion： The results of anti-tumor activity showed that the essential oil had moderate inhibitory activity against the four tumor cell lines. The inhibition ratio was above 80% for K-562, BEL-7402 and GSC-7901 cells proliferation in the high concentration sample group. The assay exhibited good inhibitory effect against K-562 and BEL-7402 cell lines with ICs0 value of 3.78, 8.14 g·L^-1 respectively, which was better than positive control mitomycin C. Meanwhile, the essential oil showed moderate inhibitory effect against SGC-7901 cell line with ICs0 of 22.94 g·L^-1. This experiment has provided scientific foundation for further utilization of P. consanguinea.

关 键 词： 沙煲暗罗 精油 气相色谱质谱联用 抗肿瘤活性