作　　者： (郭思宇）; (刘明平）; (周赛妮）; (王美静）; (赖有勤）; (柯国韩）;

机构地区： 广州中医药大学中药学院,广州510006

出　　处： 《中国实验方剂学杂志》 2017年第17期127-132,共6页

摘　　要： 目的:探讨川芎-天麻配伍对血瘀型偏头痛的治疗作用及不同配比的疗效差异性。方法:SD雄性大鼠42只,随机分为正常组(Z),模型组(M),假手术组(J),川芎-天麻(4∶1)组(A),川芎-天麻(1∶1)组(B),川芎-天麻(1∶4)组(C),舒马曲坦组(阳性组,Y)。A,B,C 3组大鼠,将川芎-天麻浓缩液按剂量20 m L·kg^(-1)·d^(-1),连续灌胃8 d,Z,J组将舒马曲坦水溶液按20 m L·kg^(-1)·d^(-1),连续灌胃8 d。大鼠冰水浴后皮下注射盐酸肾上腺素(0.8 mg·kg^(-1))和硝酸甘油(10 mg·kg^(-1))复制血瘀证偏头痛模型,采用酶联免疫吸附(ELISA)检测各组大鼠血清中降钙素基因相关肽(CGRP),血栓烷素B2(TXB_2),6-酮-前列腺素F1α(6-Keto-PGF1α)和脑组织一氧化氮(NO),一氧化氮合酶(NOS),降钙素基因相关肽(CGRP),内皮素(ET)水平。结果:与正常组比较,模型组血清中NO,NOS,CGRP,6-Keto-PGF1α含量,NO/ET明显升高(P<0.05);脑组织中NO,NOS,ET,CGRP,TXB_2,6-Keto-PGF1α含量明显升高(P<0.05);各比例组比较,川芎-天麻(4∶1)组能显著降低模型组血清中CGRP,6-Keto-PGF1α含量,NO/ET(P<0.05),提高TXB_2含量,TXB_2/6-Keto-PGF1α(P<0.05);(4∶1)组显著降低模型动物脑组织中NO,ET,TXB_2,6-Keto-PGF1α含量与NOS活性,提高TXB_2/6-Keto-PGF1α(P<0.05)。结论:川芎-天麻配伍对治疗血瘀型偏头痛具有疗效,不同配比组疗效有差异,综合多项指标考虑,川芎-天麻(4∶1)组疗效最好。 Objective： To investigate the efficacy differences of various combinations of Chuanxiong Rhizoma-Gastrodiae Rhizoma in treatment of blood stasis type migraine. Method： Total 42 SD rats were randomly divided into 7 groups： normal group （Z）, model group （M）, sham-operated group （J）, Chuanxiong Rhizoma- Gastrodiae Rhizoma groups A （ 1 ： 4） , B （4：1 ） , C （ 1 ： 1 ） , and Sumatriptan group positive control group Y）. In groups A, B, C, intragastric administration of Chuanxiong Rhizoma-Gastrodiae Rhizoma concentrated solution at 20 mL· kg^-1·d^-1 was given for continuous 8 weeks; in groups Z and J, water and sumatriptan solution at 20 mL·kg^-1·d^-1 was given respectively for 8 weeks by intragastric administration. SD rats received subcutaneous injection of hydrochloride （0. 8 mg·kg^-1 ） and trinitrin （ 10 mg·kg^-1 ） after ice bathing to establish blood stasis type margarine models. Calcitonin gene related peptide （CGRP） , thromboxane B2 （TXB2） , 6-keto-prostaglandin F1 alpha （6-Keto-PGF1α） in serum and nitric oxide （NO）, nitric oxide synthase （NOS）, CGRP, endothelin （ET） in brain tissues were detected by enzyme linked immunosorbent assay （ELISA）. Result： As compared with the normal group, the levels of NO, NOS, CGRP, 6-Keto-PGF1α and the ratio of NO/ET in serum, and the levels of NO, NOS, ET, CGRP, TXB1 and 6-Keto-PGF1α in brain tissues were increased in model group （P 〈 0.05）. As compared with the model group, NO/ET, CGRP and 6-Keto-PGF1α in serum were reduced （P 〈 0.05） in Chuanxiong Rhizoma-Gastrodiae Rhizoma 4：1 group, TXB2 content and TXB2/6-Keto-PGF1α ratio were increased （P 〈 0.05 ）. Chuanxiong Rhizoma-Gastrodiae Rhizoma 4：1 group could reduce NO, ET, TXB2 , 6-Keto-PGF1α levels in brain tissues and NOS activity （ P 〈 O. 05）, and increase the ratio of TXBJ6-Keto-PGF1α （P 〈 0. 05 ） in brain tissues. Conclusion： All the combination groups were effective for blood stasis type migraine, and grou

关 键 词： 川芎 天麻不同配比 偏头痛 血瘀证 酶联免疫吸附 酮 前列腺素