作　　者： (王燕惠）; (顾元国）; (范李萍）; (段雅洁）; (高文伟）; (曲延英）; (陈跃华）;

机构地区： 新疆农业大学农学院/新疆农业大学农业生物技术试验室,乌鲁木齐830052

出　　处： 《新疆农业科学》 2017年第8期1437-1443,共7页

摘　　要： 【目的】开发与含油量相关的分子标记,发掘与该性状相关的基因。【方法】以Springfield-B和ymnm-8作为亲本构建F2群体,利用SRAP分子标记构建甘蓝型油菜遗传连锁图谱。【结果】该遗传图谱含有52个标记位点,图谱全长1 039.6 c M,含有15个连锁群,标记间的平均遗传距离为19.9 c M。分析与含油量相关的QTL位点,获得了2个与含油量相关QTL位点。其中q OC1位于连锁群LG1:EM9ME6-EM17ME24标记区,q OC15位于连锁群LG1:EM3ME5-EM4ME22a标记区间,其表型变异解释率分别为11.23%和4.12%,加性效应值分别为0.48和3.56。【结论】获得了2个与含油量相关QTL位点,为研究甘蓝型油菜含油量奠定了一定的基础。 [Objective] To locate the molecular markers related to the oil and explore the genes related to the trait.[Method] In this study, F2 population was constructed with Springfield - B and ymnm - 8 as parents,and SRAP molecular markers were used to construct the genetic linkage map of Brassica napus L .[Result] The results showed that the genetic map contained 52 marker sites with a total length of 1,039. 6 cM and 15 linkage groups. The average genetic distance between the markers was 19. 9 cM. The QTLs associated with oil content were analyzed and two QTLs related to oil content were obtained. QOC15 was located in the linkage group LG1 ： EM3ME5 -EM4ME22a, and the explanatory rates were 11.23% and 4. 12% , respectively, and the additive effect values were 0. 48 and 3.56, respectively. The qOCl was located in the linkage group LG1 ： EM9ME6 -EM17ME24. [ Conclusion] Two QTL loci related to oil content were obtained, which laid a foun- dation for the study of oil content in Brassica napus L.

关 键 词： 甘蓝型油菜 含油量 遗传连锁图谱 定位