作　　者： (杨庆文）; (李广莹）; (丁文）;

机构地区： 深圳市宝安区中医院（集团）,广东深圳518133

出　　处： 《中国当代医药》 2017年第24期118-120,共3页

摘　　要： 目的探讨使用干式和水浴式融浆机两种方式融解冷沉淀以及融解时间长短对冷沉淀中凝血因子生物活性的影响,为达到更好的融浆效果提供依据。方法选取2016年2月3~7日我院宝安中心血站提供的120袋冷沉淀(规格1.0 U/袋),采集时间相近,均不能超过5 d,将120袋冷沉淀随机分为两组,每组60袋,编为1组和2组。1组采用干式融浆机溶解,2组采用水浴式融浆机溶解,对120份置于不同融浆机37℃融解10、20、30 min的冷沉淀样品,分别测定FⅧ、VWF抗原、PT、TT、APTT及Fib。结果两组的融浆效果及各个检测指标比较,差异无统计学意义(P>0.05)。但随着融浆时间的延长,会导致APTT延长,FⅧ、VWF抗原活性下降,差异有统计学意义(P<0.05)。结论融浆时间延长会导致凝血因子FⅧ、VWF抗原活性下降,APTT延长,为达到更好的临床输血疗效,推荐融解冷沉淀的最佳时间为10 min。 Objective To investigate the use of dry and water-bath blood soluble machine for cryoprecipitation dissolution and the effect of length of dissolution time on the biological activity of coagulation factors in cryoprecipitate,in order to provide the basis for better results.Methods Altogether 120 bags of cryoprecipitate（Specification：1.0 U/Bag）with similar blood collection dates（less than 5 days） that were provided by our Central Blood Station of Baoan from February 3rdto February 7thin 2016 were selected and randomly divided into two groups（group 1 and group 2）,with 60 bags in each group.group 1 adopted dry blood soluble machine for cryoprecipitation dissolution;while,group 2 adopted water-bath blood soluble machine for cryoprecipitation dissolution.FactorⅧ（FⅧ）,von Willebrand factor antigen（v WFAg）,prothrombin time（PT）,thrombin time（TT）,activated partial thromboplastin time（APTT） and plasma fibrinogen（Fib）of 120 cryoprecipitate samples that were placed in different blood soluble machines at 37℃ for 10,20 min and 30 min were determined.Results There was no statistically significant difference between group 1 and group 2 in the effects of blood plasma dissolution and all the indexes（P〉0.05）;however,with the prolonging of dissolution time,prolonged APTT as well as decreased activity of F Ⅷ and VWF antigen were seen,with statistically significant difference（P 0.05）.Conclusion Prolonged blood plasma dissolution time will lead to decreased activity of FⅧ and VWF antigen as well as prolonged APTT,with statistically significant difference.To achieve better efficacy of clinical blood transfusion,the best time for cryoprecipitation dissolution is recommended to be 10 min.

关 键 词： 不同融浆方式 冷沉淀凝 血因子 融浆时间