作　　者： (孙梦桐）; (马欢欢）; (吕欣然）; (林洋）; (白凤翎）; (励建荣）; (宋强）;

机构地区： 渤海大学食品科学与工程学院、辽宁省食品安全重点实验室、生鲜农产品贮藏加工及安全控制技术国家地方联合工程研究中心,辽宁锦州121013

出　　处： 《食品工业科技》 2017年第17期119-124,共6页

摘　　要： 目的:优化抑制荧光假单胞菌的复合乳酸菌组合。方法:应用牛津杯琼脂扩散法筛选对荧光假单胞菌具有协同抑制作用的复合乳酸菌,通过响应面法进行优化。结果:从11组复合乳酸菌中筛选获得Lactobacillus plantarum DY4-2、Lb.sakei YP4-5和Lb.sakei LY1-6组合的抑菌作用最强,对荧光假单胞菌的抑菌直径为25.29 mm,比DY4-2、YP4-5和LY1-6单菌株分别高出2.93、4.80和5.35 mm。利用Box-Behnken进行三因素三水平的实验设计,以抑菌直径为响应值进行分析,优化最佳条件为:菌株DY4-2、YP4-5和LY1-6复合比2∶3∶1、培养温度25℃、培养时间为48 h,优化的抑菌直径为25.84 mm。结论:乳酸菌复合可有效增加菌株间的协同作用,增加对荧光假单胞菌的抑菌活性,为研发高效控制水产品腐败菌乳酸菌生物制剂提供理论依据。 Objective： To optimize an efficient complex lactic acid bacteria （LAB）with strong antagonistic activity against Pseudomonasfluorescens.Methods： The complex LAB strains with synergic and antagonistic effects against P.fluorescens were screened using the method of Oxford cup agar diffusion. The complex LAB were optimized by response surface methodology. Results ：The best inhibitory complex of Lactobacillus plantarum DY4-2, Lb.sakei YP4-5 and Lb.sakei LY1-6 were screened from 11 groups of complex LAB against P.fluorescens, and the inhibitory zone diameter reached 25.29 mm, which was higher 2.93,4.80 and 5.35 mm than strain DY4-2, YP4-5 and YP1-6 respectively.Three-factor-three-level experiments designs were developed by Box-Behnken,and the inhibitory zone diameter was used as the responsive values.The bacterial suspension of strain DY4-2, YP4-5 and YP1-6 combined with the ratio of 2： 3： 1, culture temperature 25 ℃and culture time 48 h, which the inhibitory zone diameter against P.jquorescens was 25.84 mm in the optimum conditions. Conclusion：The complex LAB can largely increase the synergy between the strain and the inhibitory activity against P.fluorescens, which can provide theoretical basis for the research and development efficient LAB biopreservation control of P.fluorescens in aquatic products spoilage.

关 键 词： 复合乳酸菌 荧光假单胞菌 抑菌作用 响应面 优化