作　　者： (秦芸）; (石沛霖）; (刘维维）; (郭丹郡）; (何慧）;

机构地区： 华中农业大学食品科学技术学院、环境食品学教育部重点实验室,湖北武汉430070

出　　处： 《食品工业科技》 2017年第17期305-309,共5页

摘　　要： 目的:比较普通大米肽、富硒大米肽和硒代蛋氨酸的体内抗氧化活性。方法:将60只老龄(12月龄)雄性昆明小鼠和10只少龄(1月龄)雄性昆明小鼠分成7组(每组10只):老龄模型组(M组)、少龄对照组(YC组)、普通大米肽组(400 mg/kg·bw,Ⅰ组)、富硒大米肽组(400 mg/kg·bw,Ⅱ组)、硒代蛋氨酸组(2.5μg/kg·bw,Ⅲ组)、富硒大米肽+硒代蛋氨酸组(200 mg/kg·bw+1.225μg/kg·bw,Ⅳ组)和白藜芦醇组(10 mg/kg.bw,PC组),其中富硒大米肽组、硒代蛋氨酸组以及富硒大米肽+硒代蛋氨酸组中的硒含量一致,连续灌胃30 d。结果显示:与M组相比,YC组、Ⅱ组、Ⅲ组、Ⅳ组、PC组小鼠血清中超氧化物歧化酶(SOD)活性极显著提高(p<0.01);YC组、Ⅰ组、Ⅳ组的还原型谷胱甘肽(GSH)含量显著增加(p<0.05),Ⅱ组和PC组GSH含量极显著增加(p<0.01);YC组、Ⅱ组、PC组的过氧化氢酶(CAT)活性显著增加(p<0.05);Ⅲ组、Ⅳ组的肝匀浆中谷胱甘肽过氧化物酶(GSH-Px)活性显著提高(p<0.05),Ⅱ组的GSH-Px活性极显著增加(p<0.01);Ⅱ组、Ⅳ组、PC组的T-AOC显著增加(p<0.05);YC组、Ⅱ组的丙二醛(MDA)显著减少(p<0.05),PC组的MDA极显著减少(p<0.01);YC组、PC组的GSH显著增加(p<0.05),Ⅱ组的GSH极显著增加(p<0.01),以及胸腺、脾脏指数等指标均有不同程度地改善。其中所有指标以富硒大米肽组效果最好,抗氧化活性显著高于同剂量的普通大米肽,大多数指标也显著优于同等硒含量的硒代蛋氨酸组。结论:富硒大米肽中的硒与肽对增强抗氧化能力有一定的增效作用。 Objective ：To compare the antioxidant activity of the ordinary rice peptides, selenomethionine and selenium-riched peptides.Methods：The mice（ 12 months of age）were administrated by the gavage of ordinary rice peptides（400 mg/kg· bw, I group） , selenium- riched peptides （ 400 mg/kg· bw,Ⅱ group） , selenomethionine （ 2.5 μg/kg· bw, Ⅲ group） , selenium- riched peptides（200 mg/kg-bw） ＋ selenomethionine（ 1.225 txg/kg.bw, Ⅳ group）and resveratrol（ 10 mg/kg-bw,PC group）for 30 d respectively in different groups.The selenium content of Ⅱ group, ⅡI group and Ⅳ group was same. Results ： It showed that compared with the model group, YC group, 11 group, BI group, Ⅳ group, PC group showed highly significant improvement on SOD in the serum（p 〈 0.01 ） ;YC group, Igroup, Ⅳ group showed significant improvement on GSH in the serum（p 〈 0.05 ）, Ⅱ group and PC group showed highly significant improvement on GSH （p 〈 0.01 ）;YC group, 11 group, PC group showed significant improvement on CAT in the serum（p 〈 0.05 ） ; Ill group, Ⅳ group showed significant improvement on GSH-Px in liver homogenateand（p 〈 0.05 ）, Ⅱ group showed highly significant improvement on GSH-Px（p 〈 0.01 ） ; Ⅱ group, Ⅳ group, PC group showed significant improvement on T-AOC in liver homogenateand（p 〈 0.05 ） ;YC group, Ⅱ group showed significant decrease on MDA in liver homogenateand（p 〈 0.05 ） , PC group showed highly significant decrease on MDA（p 〈 0.01 ） ; YC group,PC group showed significant improvement on GSH in liver homogenateand （p 〈 0.05 ）, Ⅱ group showed highly significant improvement on GSH（p 〈 0.01 ） , and thymus and spleen index showed different degrees of improvement.The effect of selenium -riched peptides was almost the best, which the antioxidant activities of selenium-riched peptides were significantly higher than that of the ordinary rice peptides and most indexes of selenium-riched peptides were also significantly bett

关 键 词： 富硒大米肽 硒代蛋氨酸 抗氧化 增效作用