作　　者： (武志超）; (杨欢东）; (何杜鹃）; (宋晓勇）; (周化斌）; (杨海龙）;

机构地区： 温州大学生命与环境科学学院,浙江温州325035

出　　处： 《广西科学》 2017年第4期376-381,388,共7页

摘　　要： 【目的】有效地促进木耳菌体生长及胞外多糖的生产,确定可显著提高木耳液体发酵生产的促进剂及其添加量。【方法】在木耳液体发酵培养基中分别添加20种不同的促进剂,每种促进剂设置4个浓度,发酵6d后检测发酵液中的菌丝体、胞外多糖含量。然后对促进效果较好的Tween40、硬脂酸、棕榈酸、油酸进行正交试验。【结果】单因素试验结果表明,0.1%的棕榈酸、1%的硬脂酸、0.5%的Tween40、0.1%的油酸均可显著促进木耳胞外多糖的生产。正交试验结果表明,在发酵培养基中添加剂最佳组合为0.5%Tween40、1.5%硬脂酸、0.1%棕榈酸、0.25%油酸。【结论】硬脂酸、棕榈酸、油酸和Tween40可显著促进木耳胞外多糖的发酵生产。 【Objective】To effectively enhance the mycelia growth and extracellular polysaccharide production of Auricularia auricula,stimulators for liquid fermentation of A.auricula were screened.【Methods】Twenty stimulators were respectively added to the fermentation media at four different concentrations.The extracellular polysaccharide and mycelia biomass were determined after 6dof fermentation.Moreover,Tween40,stearic acid,palmitic acid and oleic acid were selected for orthogonal experiment.【Results】0.1% of palmitic acid,1% of stearic acid,0.5% of Tween40 and 0.1%of oleic acid in the culture medium significantly increased the yield of extracellular polysaccharide.Orthogonal experiment results showed that the optimal stimulator combination in the fermentation media was 0.5% Tween40,1.5% stearic acid,0.1% palmitic acid,0.25% oleic acid.【Conclusion】Stearic acid,palmitic acid,oleic acid and Tween40 could promote the extracellular polysaccharide bio-synthesis in submerged culture of A.auricula.

关 键 词： 木耳 生物量 多糖 促进剂 发酵生产