作　　者： (孙兴臣）; (熊晓妍）; (李敏婕）; (李玉峰）; (姚大伟）; (万玉萌）; (孔志龙）; (许家荣）;

机构地区： 南京农业大学动物医学院,南京210095

出　　处： 《病毒学报》 2017年第5期720-727,共8页

摘　　要： 鸽圆环病毒(Pigeon circovirus,PiCV)是圆环病毒科的一个成员,是一种免疫抑制性病原。为了研究PiCV中国流行毒株的基因特征和遗传进化关系,本研究应用PCR方法对江苏省某赛鸽公棚的22份样品进行了PiCV检测,结果有6份样品为阳性。采用重叠PCR技术得到一株鸽圆环病毒全基因序列,命名为JS15-1(GenBank登录号:KX431143)。基因序列分析表明,JS15-1全长为2 034bp,包含3个主要开放阅读框。核苷酸同源性比较结果显示JS15-1与GenBank上登录的其他鸽圆环病毒分离株的全基因核苷酸同源性为85.3%~97.1%,与比利时分离的Bel20株同源性最高。不同鸽圆环病毒Rep基因同源性为92.1%~96.6%,Cap基因同源性为72.2%~99.4%。全基因遗传进化树显示JS15-1与之前的中国分离株处于不同的分支,而与Bel20的亲缘关系最近,Rep基因进化树显示JS15-1与Bel20、PiCV/Japan/2010、Ita4B位于同一分支,Cap基因进化树显示JS15-1与Bel20处于同一分支,亲缘关系最近。氨基酸序列比较发现,JS15-1与Bel20在Rep蛋白存在6个氨基酸残基的差异,而Cap蛋白存在1个氨基酸残基的差异和1个氨基酸残基的插入。研究结果表明,JS15-1极可能来源于比利时,而且发生了变异。 The pigeon circovirus （PiCV） is a member of the genus Circovirus of the family Circoviridae. The PiCV is an immunosuppressive pathogen. In the present study, a polymerase chain reaction（ PCR） specific to the PiCV was used to detect samples collected from racing pigeons in Jiangsu Province, China. Six out of 22 samples were positive in PCRs. Total DNA was extracted from the JS15-1 sample and the genome was amplified and sequenced. Complete genome sequences were submitted to the GenBank database （Gen- Bank number KX431143）. The full-length of the genome was 2034 bp and contained three main open reading frames. Homology analyses showed that the overall nucleotide identity between this genome and others accessed in the National Center for Biotechnology Information database is ranged from 85.3 % to 97.1%. The best identity was with Bel20, in which the identity of Rep gene nucleotides ranged from 92.1% to 96.6% and that of Cap gene nucleotides ranged from 72.2% to 99.4%. A phylogenetic tree based on the full-length genome also showed that JS15-1 had the closest relationship with the Bel20 that emerged in Bel- gium in 2006. A phylogenetic tree based on the Rep gene showed that JS15-1 was located in the same branch as Bel20, PiCV/Japan/2010, and Ita4B. A phylogenetic tree based on the Cap gene showed that JS15-1 and Bel20 were in the same branch, and JS15-1 had the closest relationship with Bel20. Compared with Bel20, six amino-acid mutations were found at residues 8, 10, 53, 69, 95 and 202 in the Rep protein and one amino-acid was found at residue 33 in the Cap protein. A one amino-acid insertion was found at residue 34 in the Cap protein. These results suggest that JS15-1 may have originated from Belgium, and that JS15-1 was imported from a large number of breeders from Belgium within the last two decades.

关 键 词： 鸽圆环病毒 赛鸽 基因 遗传进化分析