作　　者： (张丽丽）; (苏松柏）; (张建玲）; (朱迪）; (汤瑾）; (黄建）;

机构地区： 贵州医科大学神奇民族医药学院,贵州贵阳550003

出　　处： 《贵阳中医学院学报》 2017年第5期35-38,61,共5页

摘　　要： 目的:建立以高效液相色谱法测定如意金黄散中盐酸小檗碱含量的方法。方法:采用高效液相色谱法,以盐酸小檗碱为对照品,色谱柱为Agilent C18(150mm×4.6mm,5μm),柱温为25℃,流动相为乙腈-0.1%磷酸水溶液(45∶55)(每100m L加十二烷基磺酸钠0.1g),流速为1.0m L·min-1,检测波长为265nm。结果:盐酸小檗碱的线性范围为0.0540~1.0800μg(R=0.9999),平均回收率为100.52%(N=3),四批样品含量测定结果均值为:本品每1g含盐酸小檗碱为0.71mg。结论:本方法专属性强,重现性佳,且稳定,可靠。 Objective：To establish the method of determinating the content of berberine hydrochloride in Ruyijinhuang Powders by HPLC. Methods： HPLC methods was used and Berberine hydroehloride was selected as reference substance. The Agilent C18 column （150mm×4. 6mm, 5μm） was used. Col ：55） （0. lg Sodium dodecyl sulfonate was added in 100mLWater）, with a flow rate at 1.0mL/min and wavelength at 265nm. Resuits： The linear range for detection of berberine hydrochloride was 0. 0540-1.0800μg （ R = 0. 9999）. The average recovery rate of the method was 100. 52% （N = 3）. The average content of the four batches of samples is： the content of berberine hydrochloride is 0. 71mg perlg. Conclusion： The method was reliable and stable with good reproducibility and strong specificity.

关 键 词： 如意金黄散 黄柏 盐酸小檗碱 含量测定 高效液相色谱法