作　　者： (马辰婕）; (吴小梅）; (林茂）; (黄力行）; (马英）; (鄢庆枇）;

机构地区： 集美大学水产学院,福建厦门361021

出　　处： 《微生物学通报》 2017年第9期2089-2095,共7页

摘　　要： 【目的】了解复合1型整合子在水产养殖环境中的分布和流行特征。【方法】对108株分离自福建水产养殖场的耐药细菌,通过PCR和序列分析,检测其复合1型整合子上下游保守区和可变区的携带情况。【结果】有86株(79.6%)和47株(43.5%)分别携带1型整合子和ISCR1元件,这两种上下游保守区均携带的耐药细菌则有26株(24.1%),其中16株(14.8%)耐药细菌成功地扩增出上下游可变区,分布于8属9种。进一步对ISCR1上下游序列的拼接和分析,表明这16株细菌携带两种类型的复合1型整合子:(1)int I1-aac(6')-Ib-cr-arr-3-dfr A27-aad A16-qac E 1-sul1-ISCR1-sdr-qnr B6-qac E 1-sul1(15株);(2)int I1-aac(6')-Ib-cr-arr-3-dfr A27-aad A16-qac E 1-sul1-ISCR1-sap A-like-qnr B2-qac E 1(truncated)-sul1(1株,肺炎克雷伯菌C12),该阵列为新发现的复合1型整合子结构。【结论】复合1型整合子在水产养殖环境中并不少见,且存在于多种细菌中,但其基因阵列结构缺乏多样性。 [Objective] In order to understand the distribution and genomic characteristics of complex class 1 integron in aquaculture environment. [Methods] 108 strains of drug-resistant bacteria were isolated from aquaculture farms in Fujian province to check the carrying status of the upstream and downstream conserved and variable regions of the complex class 1 integron by PCR and sequencing. [Results] The results showed that 86 strains （79.6%） carried class 1 integrons and 47 strains （43.5%） carried ISCR1 elements. However, only 26 strains （24.1%） have both of the elements, of which 16 strains （14.8%） were successfully amplified upstream and downstream variable regions, involving 9 species, 8 genera. Furthermore, splicing and analysis of upstream and downstream sequences indicated that these 16 strains carried two types of complex class 1 integrons： （1） int11-aac（6＇）-1b-cr-arr-3-dfrA27-aadA16-qacE△1-sull-ISCR1-sdr-qnrB6-qacE△1-sull（15 strains）; intI1-aac（6＇）-Ib-cr-arr-3-dfrA2 7-aadA 16-qacE△ 1-sul l-lSCRl-sapA-like-qnrB2-qacE△1 （truncated）-sull （only Klebsiella pneumoniae C12 strain）, which is a noval structure of complex class 1 integron. [Conclusion] The complex class 1 integron is not infrequent in aquaculture environment and exists in various bacteria, yet its gene structure lacks diversity.

关 键 词： 复合 型整合子 整合子 耐药性