作　　者： (郭琼）; (肖琳）; (于忆潇）; (王楚楚）; (缪爱军）; (宋晓骏）; (杨柳燕）;

机构地区： 南京大学环境学院污染控制与资源化研究国家重点实验室,江苏南京210023

出　　处： 《微生物学通报》 2017年第9期2019-2028,共10页

摘　　要： 【目的】探索以圆币草(Hydrocotyle verticillata)发酵液作为碳源时硫酸盐还原菌处理重金属废水的效果,以便于高效去除废水中的重金属离子。【方法】以厌氧污泥为硫酸盐还原菌接种菌群,添加大型水生植物圆币草发酵液,并以乙醇、乳酸钠、葡萄糖、蔗糖和乙酸钠为对照,测定不同碳源下硫酸盐还原效率,分析其对废水中重金属离子(Pb2+,Cd2+,Cu2+,Ni2+)的去除能力。【结果】硫酸盐还原菌能有效利用圆币草发酵液中有机物,在COD/SO42-为1.2、5.0和7.0时硫酸盐最大还原率分别为24.4%、43.6%和60.0%。以发酵液为碳源时硫酸盐还原效率高于葡萄糖、蔗糖和乙酸钠,但低于乙醇和乳酸钠。在添加圆币草发酵液的批次试验反应器中,对低浓度4种重金属离子混合废水具有良好的处理效果,Cd2+、Cu2+、Pb2+和Ni2+的去除率分别为95.2%、98.7%、93.0%和89.6%。当Cd2+、Cu2+、Pb2+和Ni2+浓度为10 mg/L时,以圆币草发酵液为碳源的批次反应器对4种重金属离子仍具有良好的处理效果,去除率均超过90%,且硫酸盐还原菌的活性没有受到抑制。【结论】大型水生植物发酵液作为硫酸盐还原菌的碳源,不仅能有效进行重金属废水的生物深度处理,而且可以实现大型水生植物的资源化。 [Objective] We studied the use of Hydrocotyle verticillata fermentation broth as carbon source for sulfate-reducing bacteria and to remove heavy metals from wastewater. [Methods] Anaerobic sludge was used as the seed of sulfate-reducing bacteria, and reactors were fed with Hydrocotyle verticillata fermentation broth and synthetic wastewater containing Pb2＋, Cd2＋, Cu2＋, Ni2＋ and sulfate. [Results] Sulfate-reducing bacteria could use the organic matter in the fermentation broth, and the sulfate reduction rates were 24.4%, 43.6% and 60.0% when the ratios of COD to SO42- were 1.2, 5.0 and 7.0, respectively. When the concentrations of Cd2＋, CuE＋, Pb2＋ and Ni2＋ were 10 mg/L, the removal rates of these heavy metals were more than 90%, and the activity of sulfate-reducing bacteria was not inhibited. [Conclusion] Hydrocotyle verticillata fermentation broth can be used as carbon source of sulfate-reducing bacteria to treat wastewater containing heavy metals.

关 键 词： 圆币草 发酵液 重金属 硫酸盐还原菌