作　　者： (潘奕欣）; (马宁宁）; (赵婧）; (赵梓含）; (庞燕）; (高娟）; (韩俭）;

机构地区： 兰州大学基础医学院病原生物学研究所,甘肃兰州730000 兰州大学第一临床医学院,甘肃兰州730000

出　　处： 《兰州大学学报（医学版）》 2017年第4期6-10,共5页

摘　　要： 目的探讨金黄色葡萄球菌、粪肠球菌、福氏志贺菌和铜绿假单胞菌在体外培养时对抗菌药物持留形成特征。方法采用微量稀释法测定4种病原菌对实验用抗菌药物的最小抑菌浓度(MIC)和最小杀菌浓度(MBC);选用10—100倍MIC的抗菌药物进行体外培养细菌的暴露实验,金黄色葡萄球菌暴露于诺氟沙星(100μg/mL),粪肠球菌暴露于氨苄青霉素(390.63μg/mL),福氏志贺菌暴露于诺氟沙星(50μg/mL),铜绿假单胞菌暴露于诺氟沙星(100μg/mL),计数培养液中的活菌数变化,分析4种病原菌体外培养时持留形成特征。结果 4种病原菌在受到高浓度抗菌药物作用后,对数生长期病原菌在数小时至3 d内全部被杀死;稳定期病原菌在药物作用1周后仍然有大量存活。结论 4种病原菌在体外培养状况下,对数生长期持留菌形成少,而稳定期形成大量持留菌。 Objective To investigate the characteristics of persistence formation of four important pathogens, including Staphylococcus aureus, Enterococcus faecalis, Shigella flexneri and Pseudomonas aeruginosa, against antimicrobial agents under cultural conditions. Methods The minimum inhibitory concentration （MIC） and minimum bactericidal concentration （MBC） of the four pathogens against the antimicrobial agents were determined by the microdilution method. The antimicrobial agents at 10 to 100 folds MIC concentrations were used to carry out exposure experiments with cultured bacteria. S. aureus, E. faecalis, S. flexneri, and P aeruginosa were exposed against norfloxacin （100 μg/mL）, ampicillin （390.63 μg/mL）, norfloxacin （50 μg/ mL） and norfloxacin （100 μg/mL） respectively. The characteristic changes in the persistence formation of the four pathogens were analyzed by counting the number of viable cells in the liquid cultures with time extension. Results Under exposure to high concentrations of antimicrobial agents, the four pathogens generally showed similar characteristics. The bacteria in the logarithmic phase could be killed by a high concentrations of anti- microbial agents in several hours to three days, while in stationary phase there were still a large number of bac- terial survival after they were exposed to the drugs for one week. Conclusion The persistence formation against antimicrobial agents under cultural conditions of S. aureus, E. faecalis, S. flexneri and P aeruginosa showed the same characteristics, indicating few persisters forming in the logarithmic phase while numerous persisters forming in the stationary phase.

关 键 词： 持留菌 金黄色葡萄球菌 粪肠球菌 福氏志贺菌 铜绿假单胞菌