作　　者： (闫婷婷）; (贺媛）; (高茜）; (白颖）; (李怡）; (赵永秀）;

机构地区： 内蒙古大学生命科学学院,呼和浩特010021

出　　处： 《分子植物育种》 2017年第8期3057-3062,共6页

摘　　要： 以马铃薯品种Favorita脱毒试管苗的叶片与茎段为外植体,在愈伤诱导培养基上培养不同时间,然后转入到添加了不同浓度6-BA与ZTR组合的芽诱导培养基上,观察其对马铃薯愈伤组织诱导及芽分化的影响。结果表明:茎段与叶片在愈伤培养基(MS+1 mg/L IAA+0.2 mg/L GA3+0.5 mg/L 6-BA)上分别培养2 d、4 d、6 d后,再转入添加不同浓度6-BA和ZTR的芽诱培养基上,均可以100%产生愈伤组织;50 d后调查,诱导2 d的叶片组织转入添加1 mg/L IAA、0.2 mg/L GA3、2 mg/L 6-BA和1 mg/L ZTR的MS诱导培养基,再生率100%,诱导6 d的叶片组织转入添加1 mg/L IAA、0.2 mg/L GA3、0.5 mg/L 6-BA和2 mg/L ZTR,分化率100%;诱导2 d的茎段组织转入添加1 mg/L IAA、0.2 mg/L GA3、3 mg/L 6-BA和1 mg/L ZTR,分化率50%。 Using potato variety Favorita virus-free plantlets＇ leaves and stem segments as explant, which are cultivated in callus induction medium in different timings, then the virus-free plantlets＇ leaves and stem segments are transplanted to bud induction medium having added different concentration of 6-BA combined with ZTR, to observe the effect for the callus induction and bud differentiation of potato. The results show that： Segments of stem and leaves which are cultivated on callus induction medium（MS ＋1 mg/L IAA＋0.2 mg/L GA3＋0.5 mg/L6-BA） for two days, four days, six days, respectively, and then transferred to shoot-inducing medium with different concentrations of 6-BA and ZTR, can form callus for 100%. After 50 days, the investigation shows that the regeneration rate is 100% for the leaves which are cultivated on callus induction medium two days, and then transferred to shoot-induction medium with 1 mg/L IAA, 0.2 mg/L GA3, 2 mg/L 6-BA and 1 mg/L ZTR. The differentiation rate is 100% for the leaves which are cultivated on callus induction medium for six days, and then transferred to shoot-induction medium with 1 mg/L IAA, 0.2 mg/L GA3, 0.5 mg/L 6-BA and 2 mg/L ZTR. The differentiation rate is 50% for the leaves which are cultivated on callus induction medium for two days, and then transferred to shoot-induction medium with 1 mg/L IAA, 0.2 mg/L GA3, 3 mg/L 6-BA and 1 mg/L ZTR.

关 键 词： 马铃薯 诱导 植株再生