作　　者： (邓霄霄）; (刘早利）; (王春台）; (刘新琼）;

机构地区： 中南民族大学生命科学学院、武陵山区特色资源植物种质保护与利用湖北省重点实验室、生物技术国家民委重点实验室,武汉430074

出　　处： 《分子植物育种》 2017年第8期2907-2911,共5页

摘　　要： 水稻稻瘟病抗性新基因OsAAA1是一种诱导型的广谱抗病基因,但OsAAA1的表达模式及调控机理未知。为了研究OsAAA1的表达模式及调控机理,通过对OsAAA1启动子序列的生物信息学分析,克隆了3个OsAAA1启动子区域的缺失体,将上述缺失体转入植物双元表达载体DX2181G中。通过菌落PCR、质粒PCR检测和质粒DNA酶切鉴定并测序确认,结果表明以上3个启动子系列缺失载体构建成功。为进一步研究OsAAA1启动子的表达模式及该基因的调控机理提供了参考依据。 Rice blast resistance gene OsAAA1 is a new inducible broad-spectrum resistance gene, but expression pattern and regulation mechanism of OsAAA1 promoter are still unknown. In order to study the expression pattern and regulation mechanism, the deletant of 3 OsAAA1 promoter regions were cloned by bioinformatics analysis and were transferred into the plant binary expression vector DX2181 G. The positive clones were verified by colony PCR, plasmid PCR detection and restriction endonuclease digestion of plasmid DNA and confirmed by Sanger sequencing. The results showed that 3 promoter deletion vectors were successfully constructed, which provided a reference for the further research on the expression pattern and regulation mechanism.

关 键 词： 水稻 基因 启动子 载体构建