作　　者： (王海涛）; (王丽）; (李树伟）; (朱梓赫）;

机构地区： 塔里木大学生命科学学院／塔里木盆地生物资源保护利用兵团重点实验室,新疆阿拉尔843300

出　　处： 《黑龙江畜牧兽医》 2017年第9期245-248,299,300,共6页

摘　　要： 为了统计昆明(KM)乳鼠背部皮肤初级和次级毛囊发育密度,观察并分析毛囊发育结构,以及对背部皮肤毛囊进行干细胞转录因子(Sox2)表达的鉴定,并初步分析Sox2在皮肤毛囊表达的意义,试验采用外科解剖法获取不同发育时间的乳鼠背部皮肤,冰冻包埋剂(OCT)包埋,制作冰冻切片及苏木精-伊红(H.E.)染色,观察统计新生小鼠不同时期背部皮肤毛囊的密度和生长发育情况,另外也采用免疫荧光染色方法鉴定Sox2在皮肤毛囊中的表达。结果表明:小鼠出生6 d内,总毛囊密度呈平稳增长,次级毛囊密度相比初级毛囊密度增长迅速,但初级毛囊发育早于次级毛囊且有髓质,第8天时初级毛囊形态结构基本发育完成;背部皮肤毛囊中Sox2的表达主要分布在毛囊毛球部和毛囊外根鞘。 To investigate the density of primary and secondary dorsal hair follicle development in KM suckling mice, follicle structure was observed and was analyzed and Sox2 expression in dorsal skin follicles was identified as well as its preliminary significance in skin follicles. Dorsal Skins of suckling mice at different developmental time were obtained via surgical anatomical method,were imbedded with OCT,and were made to be frozen sections followed by H. E. staining. The density and growth development of dorsal hair follicles were determined using statistical meth- od at different developmental time in suckling mice. Sox2 expression in dorsal skin follicles were identified using immun of luorescence method. The results showed that within 6 days after mice bern,the total density of hair follicles showed a steady growth,and a more rapid growth in secondary follicle density compared to primary follicle density ,but earlier and also with medulla in the development of primary follicle than secondary follicle. On day 8 primary follicle morphological structure had basically fully developed. Sox2 expression in dorsal skin follicles was mainly distributed in the folliclel hair bulb and outer root sheath.

关 键 词： 昆明 乳鼠 毛囊密度 毛囊结构 免疫荧光 干细胞转录因子