作　　者： (薛长晖）;

机构地区： 青岛农业大学化学与药学院,青岛266109

出　　处： 《食品工业》 2017年第9期112-114,共3页

摘　　要： 黄酮及其衍生物具有广泛的药理活性。研究了重铬酸钾与黄酮反应的最佳条件,建立重铬酸钾测定蕨麻黄酮含量的方法。结果表明:4个因素的影响次序为反应时间>反应温度>反应酸度>重铬酸钾浓度,最佳的反应条件为25 min,75℃,强酸性条件;该方法可确定线性方程为A=0.597 1-0.048 4C×10^(-5)(g/mL),相关系数为0.996,线性范围为0.00~12.2×10^(-5)g/mL,RSD为2.01%(n≥6),检出限为1.221×10~6 g/mL,加样回收率98.2%~102.5%之间,该方法可以用来测定蕨麻中黄酮类化合物的含量。 Flavonoids and its derivatives have wide pharmaceutical activities. The best conditions were investigated by the spectrophotometric. The results showed that the order of the condition was time〉temperature〉pH〉concentration of K2Cr2OT, the optimal condition was reaction 25 min, at 75℃, in strong acidity solution. The linear equation was A=0.597 1- 0.048 4C×10^-15 （g/mL） with the correlation coefficient of 0.996, and the linear range of the method was 0.00-12.2×10^-5 g/mL tea polyphenols, the RSD was 2.01% （n≥ 6）, and the recovery rate was 98.2%- 102.5%, the method could be used to determinate the content offlavonoids from the silverweed cinquefoil root.

关 键 词： 重铬酸钾 黄酮 蕨麻 吸光光度法 含量