作　　者： (杨平）; (魏建昌）; (张通）; (陈华翠）; (曾山崎）; (曹杰）;

机构地区： 510180,广州医科大学附属广州市第一人民医院胃肠外科

出　　处： 《中国医师进修杂志》

摘　　要： 目的 检测外周血循环肿瘤细胞(CTC)在不同分期结直肠癌(CRC)患者的表达情况,探讨其在结直肠癌转移早期诊断中的意义.方法 选取2014年1月至2015年12月住院治疗的CRC患者60例(Ⅱ~Ⅲ期42例,Ⅳ期18例)和直肠良性疾病患者30例.采用免疫磁珠富集分离技术和免疫荧光原位杂交技术(imFISH)检测CTC数目,同时应用电化学发光法检测癌胚抗原(CEA)的表达水平,分析CTC与CEA的相关性.结果 CRC患者外周血CTC阳性率[CTC≥1、5、10的阳性率分别为73.33%(44/60)、35.00%(21/60)、16.67%(10/60)]显著高于良性疾病对照组[13.33%(4/30)];Ⅳ期CRC患者外周血CTC的阳性率显著高于Ⅱ~Ⅲ期患者;CRC患者外周血CTC数量与血清CEA水平呈明显正相关(r=0.6652,P<0.01).结论 CRC患者CTC的表达显著高于良性疾病患者,且与临床分期密切相关,外周血CTC检测对CRC转移的早期诊断具有重要的临床意义. Objective To investigate the expression of circulating tumor cells (CTC) in peripheral blood of patients with different stages of colorectal cancer (CRC), and to evaluate its significance in early diagnosis of colorectal cancer metastasis. Methods Sixty patients with CRC (42 inⅡ-Ⅲstage and 18 in IV stage ) and 30 patients with benign rectal disease were recruited from January 2014 to December 2015. The CTC in peripheral blood was purified with Immunomagnetic Separation Technologie, and detected by immunofluorescence in situ hybridization (imFISH). The serum levels of CEA were detected by electrochemiluminescence method meanwhile. The correlation between CTC and CEA was analyzed. Results CTC positive rates in CRC patients were significantly higher than those in benign rectal disease controls. CTC positive rates inⅣstage were significantly higher than those inⅡ-Ⅲ stage. The expression of CTC was significantly correlated with CEA (r = 0.6652, P < 0.01). Conclusions The expression of CTC in CRC patients is significantly higher than that in benign rectal disease control group. It is closely related to clinical stages. Detection of peripheral blood CTC has important clinical significance in the early diagnosis of CRC metastasis.

分 类 号： [Z1]