作　　者： (黎国英）; (喻巍）; (李策）; (徐瑶）; (王梗）; (傅宏）; (刘英豪）;

机构地区： 大庆市龙南医院眼科, 黑龙江大庆,163453

出　　处： 《中华生物医学工程杂志》

摘　　要： 目的 观察黄芪多糖对糖尿病大鼠视网膜炎症因子表达水平及神经细胞氧化损伤的影响.方法 60只SD大鼠随机分为空白对照组,模型组、黄芪多糖(APS)低、中、高剂量组和盐酸二甲双胍组,每组10只.灌胃治疗2周后,采用酶联免疫吸附法检测各组大鼠眼球血清中肿瘤坏死因子-α(TNF-α)、白介素-6(IL-6)、CRP、超氧化物歧化酶(SOD)、丙二醛(MDA)的表达含量,HE染色检测视网膜组织蛋白表达变化,免疫组化检测大鼠视网膜组织内核转录因子κB(NF-κB)和谷胱甘肽过氧化物酶(GSH-Px)表达水平.结果 与空白组大鼠比较,模型组大鼠血清中TNF-α、IL-6、CRP、MDA表达含量增加,而SOD表达含量降低,P<0.05;与模型组比较,APS各剂量组及盐酸二甲双胍组血清中TNF-α、IL-6、CRP、MDA表达含量明显降低,而SOD表达含量升高(均P<0.05,APS高剂量组及盐酸二甲双胍组各指标均明显高于APS低、中剂量组(P<0.05).免疫组化染色观察显示,与空白组比较,模型组及各治疗组大鼠视网膜内NF-κB表达增加,而GSH-Px表达降低,均P<0.05,与模型组比较,APS各剂量组及盐酸二甲双胍组NF-κB表达含量降低,而GSH-Px表达含量升高,均P<0.05.结论 APS能够减轻DM大鼠视网膜内炎症反应来保护视网膜组织,同时通过增加抗氧化作用来降低视网膜神经细胞氧化损伤,其中降低NF-κB、TNF-α表达可能是视网膜保护机制之一. Objective To determine the effects of Astragalus polysaccharide (APS) on the expression of retinal inflammatory factors and neuronal oxidative damage in rats with diabetes mellitus(DM). Methods Sixty SD rats were randomly divided into the blank control group,model group,low-,medium-and high-dose APS groups and metformin hydrochloride group(n=10 each). After 2 weeks of gavage,the expression levels of serum tumor necrosis factor α(TNF-α),interleukin-6 (IL-6),CRP,superoxide dismutase(SOD)and malondialdehyde(MDA)in the eyeballs of rats were determined by enzyme-linked immunosorbent assay. The changes of the protein expression in the retinal tissues were determined by HE staining. The expression levels of nuclear transcription factorκB(NF-κB)and glutathione peroxidase(GSH-Px) in the retinal tissues were determined by immunohistochemistry. Results Compared with the blank group,the levels of serum TNF-α,IL-6,CRP and MDA in the model group increased(P<0.05),while the level of SOD decreased(P<0.05). Compared with the model group,the expression levels of serum TNF-α, IL-6,CRP and MDA significantly decreased in different doses of the APS groups and the metformin hydrochloride group,while the expression level of SOD significantly increased(all P<0.05). The indexes in the high-dose APS group and the metformin hydrochloride group were significantly higher than those in the low-and medium-dose APS groups(P<0.05). The immunohistochemical staining showed that compared with the blank group,the expression of NF-κB increased in the retinal tissues of the model group and all of the treatment groups,while the expression of GSH-Px decreased,all P<0.05. Compared with the model group, the expression levels of NF-κB decreased in different doses of the APS groups and the metformin hydrochloride group,while the expression level of GSH-Px increased,all P<0.05. Conclusion APS can protect retinal tissues by reducing the inflammatory reaction in the retina of DM rats. It can reduce the oxidative damage of retinal neurons by increasing the antioxidative effect,and the decrease of NF-κB and TNF-αexpression may be one of the mechanisms of retinal protection.

分 类 号： [Z1]