作　　者： (宋震）; (苏亚庆）; (王卉卉）; (匡少平）;

机构地区： 青岛科技大学环境与安全工程学院

出　　处： 《青岛科技大学学报（自然科学版）》 2017年第S1期30-34,共5页

摘　　要： 以胜利油田孤东和孤岛采油区内土壤作为研究的对象,测得土壤含水率(12%~24%);土壤中含盐量(0.8%~1.8%);土壤pH值(8.2~8.6);土壤中有机碳含量(0.1%~0.5%);以及采用火焰原子吸收-分光光度法测定了土壤中重金属Cu、Cr、Zn的含量,以得到土壤的理化性质数据。运用高通量基因测序技术测定了土壤中的古菌的DNA序列,结果表明该采油区土壤中的优势菌门是广古菌门(Euryarchaeota),同时石油污染使得土壤中的古菌多样性降低。分析该区域的土壤生态状况和古菌群落的生长状况,旨在为该油田开采区采用微生物修复技术来治理土壤原油污染问题提供理论依据。 In this paper,we selected the soil of the Gudong and Gudao oil extraction areas as the research objects and measured soil properties:moisture content(12%—24%);salt content(0.8%—1.8%);pH value(8.2—8.6);soil organic carbon(0.1%—0.5%)and determining content of Cu,Cr,Zn by AAS.By extracting DNA,after PCR amplification and fluorescence quantification,the DNA sequences of archaea in soil were determined by High Throughput gene Sequencing technology.After analyzing the soil archaeal diversity characteristics,it was found that the dominant species in soil was Euryarchaeota,meanwhile oil pollution could decrease the archaeal biodiversity in soil.This paper is intended to provide certain theoretical basis for the bioremediation of oil contaminated soil in that oilfield.

关 键 词： 古菌多样性 土壤 石油污染 微生物修复技术