作　　者： (钱静）; (杨培周）; (操丽丽）; (操新民）; (郑志）; (姜绍通）;

机构地区： 合肥工业大学食品科学与工程学院,安徽省农产品精深加工省级实验室,安徽合肥230009

出　　处： 《食品科学》 2017年第17期244-250,共7页

摘　　要： 为研究灵菌红素对肠道发育以及抗氧化能力的影响,以断奶应激诱导的肠道损伤大鼠为实验动物模型,将30只断奶SD雄性大鼠随机平均分成对照组、低剂量组和高剂量组,对照组大鼠每天灌胃生理盐水,低剂量组和高剂量组大鼠每天分别灌胃剂量为100、200μg/kg的灵菌红素溶液(以体质量计);喂养实验持续14 d后处死实验大鼠并取样;记录大鼠每天的体质量和采食量;称取各组织器官质量,测量小肠和结肠的长度,计算器官指数;采用苏木精-伊红染色方法观察回肠和结肠的形态结构,测量回肠绒毛高度和隐窝深度以及结肠黏膜厚度和杯状细胞数量;检测回肠和结肠组织中总抗氧化能力(total antioxidant capacity,T-AOC)、谷胱甘肽过氧化物酶(glutathione-peroxidase,GHS-Px)、超氧化物歧化酶(superoxide dismutase,SOD)活力和丙二醛的水平。结果表明:高剂量灵菌红素能够显著提高大鼠的体质量、平均日采食量(P<0.05),显著增加肝脏、脾脏和胸腺指数(P<0.05),显著增加小肠和结肠指数,增加回肠绒毛高度、绒腺比和结肠黏膜厚度(P<0.05);显著增加回肠组织中T-AOC、GHS-Px和SOD活力(P<0.05),并降低回肠组织中丙二醛含量;显著增加结肠组织中SOD活力(P<0.05),降低结肠组织中丙二醛含量。结论:灵菌红素有利于提高断奶大鼠的生长性能,提高回肠和结肠的抗氧化能力,具有缓解断奶应激诱导的大鼠肠道损伤的作用。 To investigate the effect of prodigiosin on intestinal development and antioxidant function in weaned rats,thirty male SD weaned rats,which suffered from intestinal injury induced by weaning stress,were randomly equally divided into control,low-dose,and high-dose groups.The rats in the control group were orally administered daily with sterile saline,while those in the low-dose,and high-dose groups were gavaged daily with100and200μg prodigiosin/kg body weight,respectively.The administration lasted for14days.All the rats were sacrificed after the last administration to collect tissue samples.Body weight and feed intake were recorded daily.Organ weights and the lengths of small intestine and colon were measured,and organ indexes were calculated.The morphological structures of ileum and colon were observed using hematoxylin and eosin(HE)staining.Ileal villus height and crypt depth,the thickness of colonic mucosa,and the number of goblet cells were measured.The total antioxidant capacity(T-AOC),glutathione-peroxidase(GHS-Px)and superoxide dismutase(SOD)activity,and malondialdehyde(MDA)content in ileum and colon were determined.The results showed that high-dose prodigiosin could significantly increase body weight,daily feed intake,liver,spleen,thymus,small intestine and colon indexes,ileal villus height,the ratio of villus height and crypt depth,the thickness of colon mucosa,T-AOC,GHS-Px and SOD activities in ileum,and SOD in colon(P<0.05),and decrease MDA contents in ileum and colon.In conclusion,prodigiosin is beneficial for improving the growth performance of weaned rats,and enhancing antioxidant capability in ileum and colon,as well as attenuating intestinal injury induced by weaning stress.

关 键 词： 灵菌红素 断奶大鼠 肠道发育 免疫功能 抗氧化能力