作　　者： (彭宏伟）; (郭丽华）;

机构地区： 曲阜师范大学医院药剂科,山东曲阜273165

出　　处： 《中国医院用药评价与分析》 2017年第8期1039-1041,共3页

摘　　要： 目的:建立醋制消肿止痛液中苦参碱、氧化苦参碱、羟基红花黄色素A的含量测定方法。方法:采用高效液相色谱法,色谱柱为Sinochrom ODS-BP色谱柱(250 mm×4.6 mm,5μm);流速为1.0 ml/min;柱温为30℃。测定苦参碱、氧化苦参碱采用流动相乙腈-0.2%磷酸水(V∶V=92∶8),用三乙胺调p H至6.0,检测波长为220 nm;测定羟基红花黄色素A采用流动相乙腈-0.2%甲酸水(V∶V=15∶85),检测波长为403 nm。结果:苦参碱、氧化苦参碱、羟基红花黄色素A的进样量分别在0.412~1.648、0.448~1.792、0.220~0.880μg范围内与峰面积具有良好的线性关系(r分别为0.999 5、0.999 3、0.999 4);苦参碱的平均加样回收率为98.90%,RSD为0.42%;氧化苦参碱的平均加样回收率为99.08%,RSD为0.69%;羟基红花黄色素A的平均加样回收率为99.03%,RSD为0.44%。结论:本方法操作简便、结果准确、重现性良好,可作为该制剂的质量检测标准,并为其开发应用提供了理论基础。 OBJECTIVE： To establish the method for content determination method of matrine,oxymatrine and hydroxy safflower yellow A contents in Xiaozhongzhitong vinegar liquid by HPLC. METHODS： HPLC method was adopted,the column was Sinochrom ODS-BP（ 250 mm × 4. 6 mm,5 μm）,with flow rate of 1. 0 ml/min and column temperature of 30 ℃. Matrine and oxymatrine were determined by mobile phase acetonitrile 0. 2% and phosphoric acid water（ V ∶ V = 92∶ 8）. Triethylamine was used to adjust PH value to 6. 0,and the wave length was 220 nm. Hydroxy safflower yellow A was determined with mobile phase of acetonitrile 0. 2% methanoic acid water（ V∶ V = 15 ∶ 85） and detection wavelength of 403 nm. RESULTS： The calibration curves were linear when the sample ranges of matrine,oxymatrine and hydroxy safflower yellow A were at 0. 412-1. 648 μg,0. 448-1. 792 μg and 0. 22-0. 88 μg,respectively（ r = 0. 999 5,r = 0. 999 3 and r = 0. 999 4）. The average recovery rate and RSD of matrine was at 98. 90% and0. 42%,oxymatrine was at 99. 08% and 0. 69%,and hydroxy safflower yellow A was at 99. 03% and 0. 44%.CONCLUSIONS： This method is simple,rapid,accurate and suitable for the detection of quality of Xiaozhongzhitong liquid,which can provide the theoretical basis for the development and application of Xiaozhongzhitong vinegar liquid.

关 键 词： 高效液相色谱法 醋制消肿止痛液 苦参碱 氧化苦参碱 羟基红花黄色素 含量