作　　者： (常青山）; (张利霞）; (米银法）; (沙晨霞）; (王倩丽）; (黄玥）; (赵运发）; (关俊超）; (余玉磊）; (韩晓鹏）;

机构地区： 河南科技大学林学院,河南洛阳471023

出　　处： 《核农学报》 2017年第10期2055-2062,共8页

摘　　要： 为探讨外源5-氨基乙酰丙酸(ALA)对盐胁迫下植物生长的缓解作用,以夏枯草为试验材料,研究1.25~10.00 mg·L^(-1)ALA对70 mmol·L^(-1)盐胁迫下夏枯草幼苗叶片和根系的抗氧化酶活性、叶片的叶绿素含量与光合特性的影响。结果表明,1.25~5.00 mg·L^(-1)ALA处理均可不同程度地提高盐胁迫下夏枯草幼苗叶片和根系过氧化物酶(POD)、过氧化氢酶(CAT)、抗坏血酸过氧化物酶(APX)的活性及可溶性糖含量;增加夏枯草叶片的叶绿素a(Chl a)、叶绿素b(Chl b)、总叶绿素(Chl a+b)及类胡萝卜素(Car)含量;提高净光合速率(Pn)、气孔导度(Gs)与蒸腾速率(Tr),降低胞间CO2浓度(Ci)。外源ALA可以通过提高抗氧化酶活性和渗透调节物质的含量,增强叶片的光合作用,进而缓解盐胁迫对夏枯草幼苗产生的伤害,从而提高夏枯草幼苗的抗盐能力,ALA浓度以5.00 mg·L^(-1)最佳。本研究结果为探明ALA缓解夏枯草盐胁迫危害的作用机理提供了理论依据。 In order to investigate the mitigative effect of exogenous 5-aminolevulinic acid（ALA） on plant growth under salt stress,Prunella vulgaris seedlings were exposed to different concentrations of ALA（an exogenous nitric oxide donor） from 1. 25 to 10. 0 mg·L（-1）. The antioxidant capacities in the leaves and roots,the chlorophyll content and photosynthetic characteristics in the leaves of P. vulgaris seedlings were studied under salt stress of 70 mmol·L（-1）. The results showed that 1. 25 5. 00 mg·L（-1）ALA could increase the activities of peroxidase（POD）,catalase（CAT）,ascorbate peroxidase（APX）, the content of soluble sugar, chlorophyll a（Chla）, chlorophyll b（Chlb）, total chlorophyll（Chl a ＋ b）,carotenoid,the net photosynthetic rate（Pn）,stomatal conductance（Gs） and transpiration rate（Tr）, meanwhile reduce intercellular CO2concentration（Ci） at different extents. Exogenous ALA could significantly alleviate the damages of salt stress on P. vulgaris seedlings and promote the salt resistance by improving the antioxidant enzyme activities, the content of osmotic adjustment substances and the photosynthetic capacities;furthermore,5. 00 mg·L（-1）ALA was considered to be the optimal concentration. This study provided a theoretical basis for the application of ALA in reducing the toxicity of salt stress to P. vulgaris seedlings.

关 键 词： 夏枯草 氨基乙酰丙酸 盐胁迫 光合特性 抗氧化能力