作　　者： (王亚光）; (梁慧芳）; (唐景霞）; (李睿）; (奚海林）; (刘勇）; (杨宏强）;

机构地区： 石河子大学医学院第一附属医院,新疆832008

出　　处： 《医学动物防制》 2017年第10期1018-1021,共4页

摘　　要： 目的探讨牛棒状杆菌引起的裸鼠过度角化症的临床表现及病理学特征。方法将裸鼠分为两组:正常对照组与过度角化症组,观察发生过度角化症的裸鼠在临床症状期及后临床症状期的临床表现,并通过苏木素-伊红(HE)染色法观察其病理学特征。结果过度角化症裸鼠临床症状期主要表现为裸鼠皮肤表面出现大量白色皮屑,棘层与角化层增厚,进入后临床症状期后皮屑消失,棘层与角化层增厚持续存在。过度角化症组临床症状期棘层厚度(48.11±3.67)μm,与正常对照组棘层厚度(21.45±2.72)μm差异有统计学意义(t=5.87,P=0.0 004);过度角化症组后临床症状期棘层厚度(43.75±3.06)μm,与正常对照组棘层厚度差异也有统计学意义(t=5.45,P=0.0 006)。过度角化症组临床症状期角化层(23.41±3.63)μm,与正常对照组角化层厚度(11.73±1.67)μm差异有统计学意义(t=2.93,P=0.019);过度角化症组后临床症状期角化层(24.73±2.49)μm,与正常对照组角化层厚度差异也有统计学意义(t=4.34,P=0.0 025)。结论应用临床表现与病理学检查相结合的方法可对裸鼠过度角化症做出诊断。 Objective To investigate the clinical manifestation and pathological characteristics of hyperkeratosis in nude mouses induced by Corynebacterium bovis. Methods Nude mouses were divided into two groups： normal control group and hyperkeratosis group. The clinical manifestations of hyperkeratosis nude mouses in the clinical stage and behind clinical stage were observed. Hematoxylin and eosin（ HE） staining was used to observe the pathological characteristics. Results The main clinical manifestations of hyperkeratosis were appearing a large amount of white dander on the surface of the skin,acanthosis and thickening of the cornified layer. After entering the behind clinical stage,in this period,the dander disappeared,furthermore the acanthosis and thickening of the cornified layer were persistent. There was significant difference in spinous layer thickness between the clinical stage of hyperkeratotic group（ 48. 11 ± 3. 67） μm and normal control group（ 21. 45 ± 2. 72） μm（ t = 5. 87,P = 0. 0 004）. There was also significant difference in spinous layer thickness between behind clinical stage of hyperkeratotic group（ 43. 75 ± 3. 06） μm and normal control group（ t = 5. 45,P = 0. 0 006）. There was significant difference in cornified layer thickness between the clinical stage of hyperkeratotic group（ 23. 41 ± 3. 63） μm and normal control group（ 11. 73 ± 1. 67） μm（ t = 2. 93,P = 0. 019）. There was also significant difference in cornified layer thickness between behind clinical stage of hyperkeratotic group（ 24. 73 ± 2. 49） μm and normal control group（ t = 4. 34,P = 0. 0 025）. Conclusion The clinical manifestations and pathological examination can be used to diagnose the hyperkeratosis in nude mouses.

关 键 词： 裸鼠 过度角化症 临床表现 病理学特征