作　　者： (王军）; (程少文）; (陈庆玉）; (袁伟）; (曾允富）;

机构地区： 海南医学院第一附属医院创伤医学中心,海南海口570102

出　　处： 《局解手术学杂志》 2017年第9期630-634,共5页

摘　　要： 目的探究TGF-β微泡靶向治疗对大鼠受损骨骼肌再生的修复作用。方法选择2月龄SD大鼠80只,随机分为实验组(包载TGF-β微泡)、单纯药物组、单纯微泡组(不包载药物)和空白对照组,每组20只,4组均进行超声透射操作,且时间及频率保持一致。测量受损腓肠肌收缩应力和松弛应力,观察受损腓肠肌光镜及电镜变化。结果损伤后1、5、9 d各组腓肠肌收缩应力差异无统计学意义(P>0.05),至第14天时,实验组和单纯药物组腓肠肌收缩应力明显高于其余2组,差异有统计学意义(P<0.05),实验组和单纯药物组腓肠肌收缩应力差异有统计学意义(P<0.05),在应力松弛方面上,损伤后第1天,实验组腓肠肌松弛应力均低于其余3组,差异具有统计学意义(P<0.05)。光镜显示,实验组受损部位在第9天时肌纤维数量增加明显,第14天时基本未见瘢痕形成。电镜下实验组腓肠肌细胞线粒体和肌浆网增多,新生肌细胞和卫星细胞明显多于其余3组。结论 TGF-β微泡靶向治疗能明显改善大鼠骨骼肌损伤后收缩应力及恢复应力衰减,同时能明显促进受损骨骼肌的修复和再生,减少瘢痕形成。 Objective To explore the effect of TGF-β microbubble targeting on the treatment of skeletal muscle injury in rats. Methods Eighty SD rats of 2 months old were randomly divided into experimental group（ including TGF-β microbubble） , pure drug group, microbubble group（ exclusive drug） and control group ,20 SD rats in each group. Four groups accepted ultrasonic transmission with consistent time and frequency. The contraction stress and stress relaxation of injured gastrocnemius muscle were measured, the changes of injured gastrocnemius muscle were observed by light microscope and electron microscope. Results There was no statistically significant difference in contraction stress of gastrocnemius muscle among all groups at 1,5,9 days after injury（ P 〉 0.05 ）. And at 14 days, the contraction stress of gastrocnemius muscle of the experimental group and the pure drug group were obviously higher than those of the rest two groups, the differences were significant （ P 〈 0.05 ）. The difference of gastrocnemius muscle contraction stress was statistically significant between the experimental group and the pure drug group （ P 〈 0.05 ）. For the stress relaxation of gastrocnemius muscle, the experimental group was lower than the other three groups at 1 day after injury, the differences were significant（ P 〈 0.05 ）. Light microscopy showed the number of muscle fiber in the experimental group increased obviously at 9 days after injury. There was no scarring at 14 days after injury. Electron microscope showed mitochondria and sarcoplasmic reticulum increased in the experimental group,and the new muscle ceils and satellite cells were significantly more than those of the rest three groups. Conclusion TGF-β cell targeted therapy can obviously improve the shrinkage stress of skeletal muscle in rats after injury and recover the stress decay, which can obviously promote the injury repair and regeneration of skeletal muscle, and reduce scar formation at the same time.

关 键 词： 转化生长因子 骨骼肌 损伤 再生 超声