作　　者： (张小军）; (习勇）; (封鹏）; (李杰）; (刘达）;

机构地区： 铜川市人民医院骨科,陕西铜川727000

出　　处： 《局解手术学杂志》 2017年第9期625-629,共5页

摘　　要： 目的研究PRR11基因在骨肉瘤中的表达情况,观察PRR11对骨肉瘤细胞增殖及转移的影响。方法采用免疫组化方法观察75例骨肉瘤及配对肉瘤旁组织中的PRR11蛋白表达情况;Western blot检测不同骨肉瘤细胞系中PRR11的蛋白表达水平;利用慢病毒建立稳定低表达PRR11的SaOS2细胞模型并检测低表达PRR11对骨肉瘤细胞SaOS2细胞增殖能力的影响。结果 PRR11在骨肉瘤组织中的表达明显高于肉瘤旁组织,其在骨肉瘤及对应肉瘤旁组织中的表达率分别为76.00%(57/75)和9.33%(7/75),差异有统计学意义(P<0.05)。PRR11表达与肿瘤的病理分级及淋巴结转移有关。PRR11在SaOS2、143B、U2OS、MG63这4株骨肉瘤细胞系中均有表达,并在SaOS2中表达最高。干扰SaOS2细胞的PRR11基因表达后,细胞的增殖及克隆形成能力相应降低,差异有统计学意义(P<0.05)。结论骨肉瘤的发生发展是多基因、多步骤的复杂过程,PRR11在骨肉瘤中高表达,且与肿瘤的进展相关,可作为骨肉瘤早期诊断与临床干预的新靶标。 Objective To explore the expression of PRR11 （ Proline-rich protein 11 ） in human osteosarcoma and investigate the effect of PRR11 on the proliferation of human osteosareoma cells. Methods Immunohistoehemical staining was applied to detect the PRR11 expression in 75 cases of osteosarcoma and corresponding normal tissues. Western blotting was used to examine PRR11 protein expression levels in osteosarcoma cell lines. We used siRNA to knock down the expression of PRR11 and tested the effects of PRR11 down-regulation on the proliferation in SaOS2 cells. Results PRR11 was overexpressed in osteosarcoma specimens compared to their paired normal tissues, the over expression rate of PRR11 in osteosareoma and corresponding paracaneerous tissues were 76% （57/75） and 9.33% （7/75） with statistical difference（P 〈0.05 ）. The high expression of PRR11 was correlated with tumor pathological grade and lymphatic metastasis （P 〈 0.05 ）. PRRll was expressed in 4 osteosarcoma cell lines which were SaOS2,143B, U2OS and MG63 respectively, the expression was highest in SaOS2 ceils. Silencing PRR11 inhibited cell growth as compared with control cells （P 〈 0.05 ）. Conclusion PRR11 is overexpression in human osteosarcoma and promotes its progression by enhancing proliferation. The increased expression of PRR11 in osteosarcoma is a new target for treatment and early diagnosis of human osteosarcoma patients.

关 键 词： 骨肉瘤 增殖 转移 进展