作　　者： (詹艳萍）; (张勇）; (张泽林）;

机构地区： 三峡大学第一临床医学院＆宜昌市中心人民医院,宜昌443003

出　　处： 《现代妇产科进展》 2017年第8期592-594,598,共4页

摘　　要： 目的:研究妊娠期糖尿病(GDM)对雌性子代成年以后糖代谢的影响,并初步探讨其机制。方法:造模采用8周龄健康成年雌性SD大鼠,分别与健康雄鼠交配。妊娠当天,GDM组母鼠给予35mg/kg STZ溶液腹腔注射,母鼠对照组腹腔注射缓冲液。两组雌性子代分别记为子代对照组和糖尿病后代组。酶联免疫吸附测定(ELISA)试剂盒检测血清胰岛素、血脂;糖耐量实验、胰岛素耐量实验和胰岛素释放实验检测其胰岛素敏感性;苏木精-伊红染色法(HE)检测胰岛形态学,q PCR检测胰腺microRNA表达。结果:糖尿病后代组大鼠比子代对照组表现轻微的胰岛素抵抗。相对于子代对照组,糖尿病后代组胰岛面积减少,miRNA-130a表达降低。结论:母亲GDM可能是诱发子代糖代谢紊乱的一个高风险因素。 Objective： To investigate the effect of maternal gestational diabetes mellitus on glycometabolism of female offspring in SD rat. Methods： Sprague-Dawley rats（ 8-week-old）were used in this study.Maternal gestational diabetes mellitus was induced by a single injection of streptozotocin（ STZ） on the first day of gestation in Sprague-Dawley rats. Control rats were injected with citrate buffer.Serum insulin and lipids were determined by enzyme linked immunosorbent assay（ ELISA）. Islet area were evaluated through haematoxylin and eosin staining.Glucose and insulin tolerance tests（ GTT and ITT） and insulin releasing test（ IRT） were performed.microRNAs expression were evaluated through q PCR in islet. Results： Compared with offspring control group,diabetes offspring group showed more slight impairment in GTT and ITT.In addition,they showed bigger islet throughout the study.The miRNA-130 a expression was decreased in diabetes offspring group.Conclusion： Maternal gestational diabetes mellitus may be a high-risk factor of glycometabolism disturbance in female offspring.

关 键 词： 妊娠期糖尿病 大鼠 子代 糖代谢