作　　者： (张晓威）; (李璐瑶）; (史晓明）; (王颖）; (魏瑗）; (原鹏波）; (赵扬玉）;

机构地区： 北京大学第三医院妇产科,北京100191

出　　处： 《现代妇产科进展》 2017年第8期569-573,共5页

摘　　要： 目的:探讨合并脐血流异常对选择性宫内生长受限(s IUGR)脐血血浆代谢组学的影响。方法:纳入6对合并脐血流异常及3对脐血流正常(对照组)的s IUGR双胎,利用气相色谱-飞行时间质谱技术(GC-TOF/MS)对双胎脐血进行代谢物检测,通过主成分分析(PCA)和正交偏最小二乘判别分析(OPLS-DA)对质谱数据进行多元变量模式识别分析,根据模型的变量投影重要性、学生t检验筛选可能的标记物,并进行代谢通路分析。结果:合并脐血流异常s IUGR筛选出17种有统计学意义的差异代谢物。其中谷氨酸、草酸、环亮氨酸、N-(2-羟乙基)亚氨基二乙酸及L-犬尿氨酸在s IUGR合并脐血流异常组增加,天冬氨酸、N-乙酰-L-天冬氨酸、乳酸、L-苏糖、酪氨酸、甘氨酸、富马酸、β-甘露糖基甘油酸、β-丙氨酸、亚油酸、棕榈酸和马来酰胺酸则降低。结论:GC-TOF/MS方法证实合并脐血流异常的s IUGR代谢组学发生改变,主要体现在脂质、碳水化合物、氨基酸、能量、核酸、辅助因子和维生素代谢等方面。 Objective： To analyze the correlation between umbilical artery（ UA）Doppler flow and selective intrauterine growth restriction（ s IUGR） cord blood metabolomics.Method： Totally 6 s IUGR with abnormal UA Doppler flow and 3 normal controls were enrolled.Gas chromatography-Time-of-flight mass spectrometry（ GC-TOF/MS） was performed on cord blood. Pattern recognition by multivariate analysis including principal component analysis（ PCA） and orthogonal projections to latent structures-discriminant analysis（ OPLS-DA） was applied.Several metabolites different between two groups were identified by variable importance in the projection values and Student＇s t-test,and statistically significant metabolites were subjected to metabolic pathways analysis.Result： Seventeen metabolites were identified.Five variables,including glutamic acid,oxalic acid,cycloleucine,N-（ 2-hydroxyethyl）-iminodiacetic acid,Lkynurenine,increased significantly,while aspartic acid,N-acetyl-L-aspartic acid,lactic acid,LThreose,tyrosine,glycine,fumaric acid,beta-Mannosylglycerate,beta-Alanine,linoleic acid,palmitic acid and maleamate were significantly decreased in s IUGR with abnormal UA Doppler flow.Conclusion： GC-TOF/MS can be used to characterize changes in metabolomics of s IUGR with abnormal UA Doppler flow,our data also suggest that metabolic pathways including lipid,carbohydrate,amino acid,energy,nucleotide,as well as cofactors and vitamins metabolism changed.

关 键 词： 单绒毛膜双胎 选择性宫内生长受限 脐动脉多普勒血流 代谢组学 气相色谱 飞行时间质谱