作　　者： (何利珍）; (钟雪云）;

机构地区： 广州医科大学附属肿瘤医院,广东广州510095

出　　处： 《实用医院临床杂志》 2017年第5期27-30,共4页

摘　　要： 目的比较人胶质瘤细胞SWO38及其克隆亚系SWOZ1和SWOZ2细胞株的PTEN(与张力蛋白同源在10号染色体有缺失的磷酸酶基因)基因表达的差异,探讨胶质瘤异质性的分子机制。方法应用RT-PCR技术、PCR-SSCP法及测序分析方法从定量和定性两方面比较PTEN基因在胶质瘤细胞SWO38及克隆亚系中表达的差异。结果 PTEN基因在SWO38表达量最多,SWOZ2次之,SWOZ1表达量最少;三株细胞的PTEN基因未发现有基因突变,其序列长度、碱基组成及排列顺序完全相同,经BLAST比对后发现与数据库中4条序列完全匹配,4条序列均为野生型PTEN的mRNA序列。结论人胶质瘤细胞SWO38及SWOZ1、SWOZ2中,PTEN基因在mRNA水平表达量明显不同,这些差异表达可能是决定两亚株细胞表现不同生物学行为的机制之一;三株细胞的PTEN基因均表现为野生型PTEN基因编码序列,提示可能是相同遗传背景的表现之一;SWOZ1和SWOZ2两株细胞可为进一步研究胶质瘤异质性分子机制提供良好的体外实验模型。 Objective To compare the differences in expression of PTEN gene,that is homologous to tension protein phospha- tase gene and missing on the I0th chromosome,in human glioma cell line SWO38 and its two sublines SWOZ1 and SWOZ2 in order to explore the molecular mechanism of tumor heterogeneity of human glioma. Methods The differences in quantitative and qualitative PTEN expression in the cell lines were examined by using RT-PCR technique, PCR-SSCP technique and sequence method. Results The levels of PTEN gene expression in the line SWO38 were highest followed by SWOZ2 and SWOZ1 was the lowest. There was no mu- tation in PTEN gene in the three cell lines. The sequences of PTEN gene in the three cell lines were the same in length, nucleotide con- stitution and alignment that completely matched with four sequences existed in the international nucleotide database by BLAST, that were mRNA sequence of wild-type PTEN. Conclusion There is a definite difference in expression of PTEN mRNA in the three cell lines. It suggests that these different gene expressions relate to the different biological characters of those two sublines. The PTEN gene in the three cell lines held the wild-type PTEN and held one of the aspects of identical hereditary background. The SWOZ1 and SWOZ2 are patent as an ideal model in study of the molecular mechanism of the heterogeneity in glioma.

关 键 词： 胶质瘤 异质性 基因表达