作　　者： (吕红艳）; (杨李红）; (任朋顺）; (张芳）;

机构地区： 邯郸市妇幼保健院新生儿科,河北邯郸056001

出　　处： 《中国医药导刊》 2017年第8期757-760,共4页

摘　　要： 目的:观察喂养不耐受对极低出生体重儿肠道菌群的影响。方法:选择30例喂养不耐受的住院极低出生体重儿为观察组,同时选择30例喂养耐受的极低出生体重儿为对照组,于生后2 d、出现喂养不耐受后2 d和喂养不耐受消失后5 d分别留取患儿大便标本,采用实时荧光定量PCR技术检测标本中的肠道乳酸杆菌及双歧杆菌。结果 :喂养不耐受组中乳酸杆菌的数量分别为[(4.15±0.25)cfu/g,(5.39±0.53)cfu/g,(7.32±0.80)cfu/g],对照组分别为[(4.17±0.27)cfu/g,(7.84±0.35)cfu/g,(7.61±1.35)cfu/g],双歧杆菌的数量分别为[(4.35±0.23)cfu/g,(5.01±0.48)cfu/g,(7.12±1.22)cfu/g],对照组分别为[(4.37±0.22)cfu/g,(8.52±0.23)cfu/g,(7.40±1.33)cfu/g]。两组于出现喂养不耐受后2 d,两种细菌数量比较差异具有统计学意义(P<0.05),而喂养不耐受出现之前和消失后5d两组细菌数量比较差异无统计学意义(P>0.05)。结论:喂养不耐受对极低出生体重儿肠道菌群的变化有明显影响。喂养不耐受使肠道双歧杆菌和乳酸杆菌数量降低。 Objective：To study the impact of feeding intolerance on intestinal flora of very low birth weight infants.Methods：Study group including 30 very low birth weight infants of feeding intolerance, and 30 feeding tolerance preterm infants were control group. At two days after birth, after clinical manifestations of feeding intolerance appeared 2 d, after the recovery of feeding intolerance 5 d, using real-time PCR technology to measure intestinal specimens lactobacillus and bifidobacterium.Results：At three periods, numbers of intestinal lactobacilli in feeding intolerance group were [ （4.15±0.25）cfu/g, （5.39±0.53） cfu/g, （7.32±0.80） cfu/g]; in the control group were [（4.17±0.27）cfu/g,（7.84±0.35） cfu/g,（7.61±1.35） cfu/g].Numbers of intestinal bifidobacteria were [（4.35±0.23）cfu/g,（5.01±0.48） cfu/g,（7.12±1.22） cfu/g]; in the control group were [（4.37±0.22）cfu/g,（8.52±0.23） cfu/g,（7.40±1.33） cfu/g]. There was statistically significant difference between the feeding intolerance group and the control group when feeding intolerance occurred 2 d（P〈0.05）. There was not statistically significant difference between the feeding intolerance group and the control group before the appearance of tolerance and after the recovery of feeding intolerance 5 d（P〉0.05）.Conclusion：Feeding intolerance is an important factor affecting intestinal microbiota in very low birth weight infants.The numbers of intestinal specimens lactobacillus and bifidobacterium were significantly lower.

关 键 词： 喂养不耐受 肠道菌群 极低出生体重儿