作　　者： (高杨）; (何则平）; (王锐）; (李艳丽）; (赵欣）; (张策）;

机构地区： 山西医科大学,山西太原030001

出　　处： 《中国医学创新》 2017年第26期25-28,共4页

摘　　要： 目的:观察CFA致炎大鼠脊髓腰膨大组织内miRNA-133b-3p与P2X4受体表达的相关关系。方法:大鼠足底注射CFA后,0 h、2 h、1 d、3 d检测大鼠机械痛和热痛的痛阈值变化,且在这四个时间点取脊髓腰膨大组织,提取总RNA和蛋白质,检测miRNA-133b-3p表达量的变化,同时检测P2X4受体m RNA和蛋白的表达量的变化。结果:与对照组相比,大鼠脚掌注射CFA后,机械痛和热痛痛阈均明显下降(P<0.05),在2 h时痛阈最低,1 d和3 d时较2 h略有回升;腰膨大部位miRNA-133b-3p表达量在2 h时没有变化,1 d和3 d开始呈现逐渐下降的趋势(P<0.05),四个时间点P2X4受体m RNA表达量比较差异均无统计学意义(P>0.05),但P2X4受体蛋白在3 d时表达量显著升高(P<0.05)。结论:CFA致炎大鼠脊髓腰膨大组织miRNA-133b-3p表达下调,同时P2X4受体表达上调,两者可能存在负向调控关系。 Objective： To observe the expression pattern of miRNA-133b-3p and P2X4R in lumbar spinal cord of complete Freund＇s adjuvant （CFA） -induced inflammatory pain rats.Method： CFA was intraplantar injected to the rats.Then we measured the threshold to mechanical stimuli and noxious thermal stimuli at 0 h, 2 h, 1 d and 3 d after CFA injection.After the behavior observation, the lumbar spinal cord were isolated from the rats, and the total RNA and protein were extracted for the detection of miRNA-133h-3p, P2X4R mRNA and P2X4R protein.Result： Compared with the control group, the threshold of rats to mechanical stimuli and noxious thermal stimuli decreased and peaked at 2 h after CFA injection （ P〈O.05 ）, then recovered slightly at 1 and 3 d （ P〈O.05 ） . The expression of miRNA-133b-3p did not change at 2 h, and decreased gradually at 1 and 3 d after CFA injection （ P〈O.05 ） .P2X4R mRNA expression had no significantly change after CFA injection （ P〉0.05 ）, but P2X4R protein increased significantly at 3 d after CFA injection （P〈O.05） .Conclusion： The expression of miRNA- 133b-3p is decreased in lumbar spinal cord of CFA-injected inflammatory pain rats, meanwhile, the expression of P2X4R protein is increased.There may be a negative relationship between miRNA-133b-3p and P2X4R.

关 键 词： 慢性痛 腰膨大 受体