作　　者： (张莉）; (伍靖）; (李晓宇）;

机构地区： 深圳市龙华区人民医院,广东深圳518109

出　　处： 《贵州医科大学学报》 2017年第8期902-905,914,共5页

摘　　要： 目的:观察AFAP1-AS1在舌鳞癌组织中的表达情况及其对舌癌细胞增殖的影响。方法:收集手术切除的舌鳞状细胞癌(TSSC)组织及癌旁0.5cm外正常组织各3例,利用实时荧光定量PCR(qRT-PCR)技术检测两种组织中AFAP1-AS1的表达;利用AFAP1-AS1的siRNA转染人舌癌细胞(Tca8113)后,qRT-PCR检测AFAP1-AS1表达,MTS实验检测Tca8113细胞的增殖能力,流式细胞术检测Tca8113细胞周期。结果:舌鳞癌组织中,AFAP1-AS1的表达明显高于癌旁组织;siRNA转染能下调Tca8113细胞AFAP1-AS1的表达,AFAP1-AS1表达沉默后,舌癌细胞的增殖能力减弱,且细胞周期阻滞。结论:AFAP1-AS1在舌癌组织中高表达促进舌癌细胞的增殖,有望成为舌癌治疗的新靶点。 Objective： To investigate AFAP1-AS1 expression in tongue squamous cell carcinoma（ TSCC） and the effect of AFAP1-AS1 on the proliferation of tongue cancer cell. Methods： Fresh tissue samples of 3 patients with tongue squamous cell carcinomas were collected as TSSC group,while 3 cases of normal tissue adjacent to carcinoma were taken as the normal control group. The expression of AFAP1-AS1 in the two groups was detected by real-time quantitative PCR（ qRT-PCR）. After tongue cancer cells were transfected with siRNA of AFAP1-AS1,MTS assay was used to examine the proliferation ability of human tongue cancer cell. And flow cytometry was applied to detect cell cycle. Results：The expression of AFAP1-AS1 in TSCC group was significantly higher than in normal control group.AFAP1-AS1 silence inhibited the proliferation of tongue cancer cell and induced cell cycle arrest,the difference was statistically significant（ P〈0. 05）. Conclusion： AFAP1-AS1 in the expression of TSCC tissue could significantly promote the proliferation of tongue cancer cell,which made a promising new therapeutic target for tongue cancer.

关 键 词： 长链非编码 舌肿瘤 癌 鳞状细胞 细胞增殖 干扰