作　　者： (李淑贤）; (周世雄）; (王森）; (郭洪胜）;

机构地区： 广东医科大学基础医学院,广东东莞523808

出　　处： 《影像研究与医学应用》 2017年第7期168-170,共3页

摘　　要： 目的 :探讨IL-6基因干扰对宫颈癌细胞Hela Hela细胞增殖的影响。细胞增殖的影响。方法 :实验分为对照组(NC)、si IL-6(NC)、si组.Real.Real time PCR检测RNA表达,CCK-8细胞增殖检测试剂盒检测细胞增殖。细胞增殖检测试剂盒检测细胞增殖。结果 :IL-6 si RNA序列转染后,si IL-6组IL-6 m RNA水平下降,约为40%。与对照组相比,在24h,48h,72h si IL-6组细胞吸光度值均下降,分别为0.38,0.38,0.55,0.9。根据细胞吸光度值计算的细胞增殖抑制率在24h,48h,72h分别为:15.5%(:15.5%(P<0.05),23.6%(P<0.05),25.6%(P<0.05)。0.05)。结论 :IL-6 R NA干扰后可抑制宫颈癌Hela细胞增殖。 Objective To investigate the effect of IL-6 gene interference on the proliferation of cervical cancer cell line, Hela. Methods The experiment was divided into the control group （NC） and the siIL-6 group.RNA level was detected by Real-time PCR, and cell proliferation was detected by CCK-8 cell proliferation test kit Results After transfection of IL-6 siRNA sequences, the level of IL-6 mRNA in siR-6 group was decreased by about 40%. Compared with the control group, absorbance at 24 h, 48 h, and 72 h of siR-6 group was decreased to 0.38, 0.55, 0.90, respectively. The related rates of cell proliferation inhibition calculated according to the absorbances were 15.5% （P〈0.05）, 23.6% （P〈0.05）, 25.6% （P〈0.05）, respectively Conclusion IL-6RNA interference can inhibit the proliferation of cervical cancer Hela cells.

关 键 词： 细胞 增殖