作　　者： (赖宝春）; (吴顺章）; (郑春明）; (王家瑞）;

机构地区： 福建省漳州市农业科学研究所,福建漳州363005

出　　处： 《果树学报》 2017年第9期1178-1184,共7页

摘　　要： 【目的】确定引起琯溪蜜柚炭疽病的病原种类,为针对性地防治炭疽病提供理论依据。【方法】2014—2015年从平和县4个乡镇采集发病材料,对病原菌进行分离、纯化、致病性测定以及形态特征观测、r DNA-ITS序列分析,对病原菌的种类进行鉴定。【结果】经单孢分离后,所获得的菌株对琯溪蜜柚果实可致病,并产生典型症状,对病原菌进行显微形态观测,病原菌分生孢子及附着孢的形态为炭疽菌属真菌,以菌株GX(3)的DNA为模板,扩增得到全长为600 bp的DNA片段,并获得该菌的rDNA-ITS序列,发现其与胶孢炭疽菌Colletotrichum gloeosporioides Penz的相似性高达100%。【结论】根据病害症状、病菌的形态特征及ITS序列同源性比较的结果,将琯溪蜜柚炭疽病的病原菌鉴定为胶孢炭疽菌C.gloeosporioides。 [ Objective] Honey pomelo is one type of perennial woody tree, which is widely planted in Pinghe county of Fujian province. With the development of the honey pomelo industry, more and more species were produced and their planting area increased year by year, and the diseases associated with honey pomelo increased gradually. Anthracnose is one of the main diseases in honey pomelo production. The disease causes up to 60% of damages to leaves and branches in production fields. Three species have been reported as causal agents of citrus anthracnose： Colletotrichum gloeosporioides, C. acutatum and C. truncatum. In this study, we identified the Colletotricbum spp. causing honey pomelo anthracnose, and aimed to deter- mine whether the same or different species occur in honey pomelo nurseries and production fields in Ping- he, and supply a theory as the basis for future scientific control of this disease. [Methods]Investigated the prevalent situation of the Guanxi honey pomelo anthracnose in the fields from 2014 to 2015. The strains were collected from Banzai town, Guoqiang township, Anhou town and Xiazhai town in Pinghe county of Fujian province. We identified the pathogen on the unhealthy honey pomelo through morphology and recorded the spore. One centimeter disks of the diseased honey pomelo leaf and branch were collected from the field and were cut and surface-sterilized with 75% ethanol and 1%0 HgC12. The disks were placed on a potato sucrose agar （PSA） medium in order to establish cultures at a temperature of 25 ℃ for 7 d. Through separating the pured pathogen single spores, to confirm the Colletotrichum spp. isolates as the causative pathogen, healthy honey pomelo fruit were sprayed with a conidial suspension （approximately 1.0 × 10^6 CFU- mL-1） of the isolates and incubated at 25 % and 90% relative humidity with plastic and incurring natural cycles of 12 h light and 12 h darkness. Four days after inoculation, lesions started to form on the honey pomelo fruit which were sprayed with the putative Colle

关 键 词： 琯溪蜜柚 胶孢炭疽菌 致病性 序列