作　　者： (苏晓菊）; (董诗琦）; (李貌）; (赵九龙）; (满晓华）; (金晶）; (李兆申）; (邹多武）; (黄浩杰）;

机构地区： 第二军医大学长海医院消化内科,上海200433

出　　处： 《中华胰腺病杂志》 2017年第4期224-227,共4页

摘　　要： 目的检测急性坏死性胰腺炎（ANP）大鼠胰腺组织高迁移率族蛋白B1（HMGB1）mRNA表达量，观察血清HMGB1水平的变化规律。 方法采用腹腔注射20% L-精氨酸250 mg/100 g体重2次、间隔1 h的方法制备ANP大鼠模型，以腹腔注射等容积生理盐水作为对照组。注射后6、18、24、36、48、72、96 h分批处死大鼠，取血检测血清淀粉酶及HMGB1水平；取胰腺组织常规行病理学检查；采用实时PCR方法检测胰腺组织HMGB1 mRNA表达量，蛋白质印迹法检测胰腺组织HMGB1蛋白表达。 结果血清淀粉酶水平自造模后6 h开始升高，18 h达峰值[（5 070±603）U/L]，48 h后恢复至正常水平，其中ANP 6、18 h时的淀粉酶活性显著高于对照组的（1 844±181）U/L（P值均〈0.05）。血清HMGB1水平自造模后6 h开始升高，36 h达峰值[（288.5±42.1）μg/L]，以后逐渐下降，ANP组所有时间点的HMGB1水平均显著高于对照组的（31.6±10.1）μg/L，差异均有统计学意义（P值均〈0.05）。ANP各时间点组的胰腺病理评分均显著高于对照组的0.38±0.52，差异有统计学意义（P值均〈0.05）。ANP 6、18、24、36、48、72、96 h组胰腺组织HMGB1 mRNA表达量分别为1.23±0.25、2.60±0.46、3.23±0.34、4.77±0.66、2.88±0.56、2.05±0.20、1.33±0.28，均显著高于对照组的0.44±0.09，其中ANP 36 h组的表达量显著高于其他各时间点组（P值均〈0.05）；ANP组6、18、36、72 h胰腺组织HMGB1蛋白表达量分别为1.14±0.02、1.15±0.01、1.22±0.01、1.22±0.04，均显著高于对照组的表达量1，其中ANP 36、72 h组的表达显著高于其他各时间点组，差异均有统计学意义（P值均〈0.05）。 结论HMGB1可能是参与ANP时全身炎症反应的晚期炎症因子之一。 Objectives To detect the expression of serum high mobility group box-1（HMGB1） and explore its changes in rats with acute necrotizing pancreatitis （ANP）. MethodsIntraperitoneal injection of 20%L-arginine in the dosage of 250 mg/100 g twice every 1 hour was used to establish ANP rat model. Intraperitoneal injection of normal saline solution in equal volume was performed in control rats. Rats were sacrificed at 6 h, 18 h, 24 h, 36 h, 48 h, 72 h and 96 h after injection. Blood samples were collected to detect serum amylase and HMGB1 level. Pancreatic tissue was collected for pathological examination. Real-time PCR was applied to detect the mRNA expression of HMGB1 in pancreatic tissue. Werstern blot was used to determine HMGB1 protein expression in pancreatic tissue. ResultsSerum amylase level began to increase at 6 h after modeling, reached the peak at 18 h [（5 070±603）U/L] and returned to normal level after 48 h. Serum amylase activity at 6 h and 18 h in ANP group was much higher than that in control group （1 844±181）U/L（P〈0.05）. The expression of HMGB1 began to increase at 6 h, reached to the peak at 36 h[（288.5±42.1）μg/L], and then decreased gradually. HMGB1 expressions at each time point in ANP group were significantly higher than those in control group （31.6±10.1）μg/L], and the differences were statistically significant （all P〈0.05）. Pathological scores in pancreatic tissues in ANP group were higher than those in control group 0.38±0.52, and the differences were statistically significant （P〈0.05）. HMGB1 mRNA expressions at t 6 h, 18 h, 24 h, 36 h, 48 h, 72 h and 96 h in ANP group were 1.23±0.25, 2.60±0.46, 3.23±0.34, 4.77±0.66, 2.88±0.56, 2.05±0.20, 1.33±0.28, which were significantly higher than those in control group 0.44±0.09, and the relative expression of HMGB1 in ANP group at 36 h was significantly higher than those at other time points （all P〈0.05）. HMGB1 protein expression in pancreatic tissue in ANP group at 6 h, 18 h, 36 h, 72 h were

关 键 词： 胰腺炎 急性坏死性 糖基化终产物 高迁移率族蛋白 炎症 大鼠