机构地区: 山西大学生命科学学院,山西太原030006
出 处: 《山西农业科学》 2017年第9期1430-1434,共5页
摘 要: 采用4种极性溶剂(乙酸乙酯、正己烷、氯仿、乙醇)通过一次提取和双溶剂萃取2种不同方法提取无花果干果中的总酚和总黄酮,测定其含量,并通过还原能力及羟自由基清除能力测定对其抗氧化活性进行比较分析。结果表明,一次提取法得到的无花果干果提取物中总酚、总黄酮含量和相应的抗氧化能力均高于双溶剂萃取法;采用同种提取方法时提取溶剂极性越大其多酚和黄酮含量与对应的抗氧化活性越高(乙醇提取物>乙酸乙酯提取物>氯仿提取物>正己烷提取物);采用60%乙醇作为提取溶剂通过一次提取法提取效果最好,提取物中总酚和总黄酮含量相对其他有机溶剂最高,相应的还原能力和羟自由基清除率均最好。 Four polar solvent (ethyl acetate, n-hexane, chloroform, ethanol) were used to extract the phenols and flavonoids from dried fig fruits by the two different methods of one-time extraction and dual-solvent extraction, the total content of which were measured. At the same time, the antioxidant activity wes compared by the scavenging activities against hydroxyl and reducing capacity evaluated. The results showed that the content of total phenols and flavonoids by one-time extraction were higher than dual-solvent extraction, also with their antioxidant activity correspondingly. Using the same extraction method, the polarity of solvent extracts was gTeater, total phenols and flavonoids content of four different extracts was higher, the same as the trend of their antioxidant activity (ethanol extract 〉 ethyl acetate extract 〉 chloroform extract 〉 n-hexane extract). The extraction effect was the best when using 60% ethanol as extraction solvent through one-time extraction method, with the highest of the total phenols and flavonoids content and their scavenging activities against hydroxyl and reducing capacity.