作 者: (孙锦霞); (黄钟); (王瑞); (翁林军); (李亚旭);
机构地区: 深圳大学医学部,广东深圳518060
出 处: 《深圳大学学报(理工版)》 2017年第4期358-363,共6页
摘 要: 采用小干扰核糖核酸(siRNA)介导Hela细胞、原代巨噬细胞和RAW264.7细胞中FBW7基因沉默,研究其对NF-κB信号通路、细胞因子表达、巨噬细胞吞噬和杀伤细菌能力的影响.通过流式细胞术、抗生素保护试验、实时荧光定量聚合酶链式反应和报告基因的研究方法,发现FBW7沉默不具有调控RAW264.7细胞吞噬细菌的能力,但能显著抑制原代巨噬细胞和RAW264.7细胞的杀菌能力及一氧化氮合成酶(nitric oxide synthase,iNOS)表达,抑制Hela中NF-κB报告基因活性,降低Hela细胞中TNFα、IL-1β和IL-6,以及原代巨噬细胞、RAW264.7细胞中MCP-1和IL-1β表达.结果表明,FBW7沉默可通过影响NF-κB活性抑制巨噬细胞对细菌的杀伤能力. SiRNA-mediated FBW7 silence in Hela,primary macrophage and RAW264. 7 were performed to assay the role of FBW7 on NF-κB activity,cytokines expression and phagocytosis and bactericidal activity of macrophage.Flow cytometry( FCM),antibiotic protection assay( APA),real time quantitative polymerase chain reaction( RT-qPCR) and report gene analysis suggest that FBW7 silence does not affect phagocytosis of RAW264. 7,but significantly inhibits bactericidal activity and nitric oxide synthase( iNOS) expression in primary macrophage and RAW264. 7,suppresses NF-κB report gene activity in Hela,decreases expression of TNFα、IL-1β、IL-6 in Hela and MCP-1、IL-1β in primary macrophage and RAW264. 7. Thus,FBW7 silence might inhibit bactericidal activity of macrophage by regulating NF-κB activity.