作　　者： (付爽）; (沈宁燕）; (倪辉）; (李利君）; (杨秋明）; (肖安风）;

机构地区： 集美大学食品与生物工程学院,福建厦门361021

出　　处： 《集美大学学报（自然科学版）》 2017年第4期20-27,共8页

摘　　要： 将法夫酵母(Phaffia rhodozyma)JMU-MVP14菌株在7 L发酵罐中进行培养,考察在不同葡萄糖质量浓度条件下,补加乙醇对法夫酵母JMU-MVP14菌株的生长和虾青素合成的影响。结果表明:低糖质量浓度下(20 g/L),恒定乙醇浓度发酵明显提高虾青素产量和细胞产率,与分批发酵相比,虾青素产量和细胞产率分别提高了27%和40%,分别达到102.8 mg/L和5.9 mg/g;中糖质量浓度下(30 g/L),恒定乙醇浓度发酵,得到较高的虾青素产量为118.4 mg/L,与分批发酵相比提高了34%;高糖质量浓度下(60 g/L),发酵得到较高虾青素产量为280.6 mg/L,是低糖质量浓度培养下虾青素产量的3.5倍,恒定乙醇发酵与分批发酵相比,生物量和虾青素产量均有显著降低。 Phaffia rhodozyma strain JMU-MVP14 was cultured in 7 L fermentor to investigate the effect of ethanol supplementation on cell growth and astaxanthin synthesis under different concentrations of glucose. The results showed that astaxanthin content and cell yield reached 102.8 mg/L and 5.9 mg/g at low concen- tration of glucose （20 g/L） under the condition of constant ethanol concentration. Compared with batch fer- mentation, astaxanthin content and ceX1 yield were significantly increased by 27% and 40%, respectively. Under the condition of 30 g/L glucose at constant ethanol concentration, the content of astaxanthin was 118.4 mg/L, which was 34% higher than that of batch fermentation. At high glucose concentration （60 g/L）, the yield of astaxanthin was 280. 6 mg/L, which was 3.5 times of the yield of astaxanthin at low sugar concentration （20 g/L）. The biomass and the yield of astaxanthin were significantly decreased by con- stant ethanol-fed fermentation compared with batch fermentation.

关 键 词： 法夫酵母 虾青素 乙醇 补料