作　　者： (殷杰）; (胡南）; (李乐）; (谢红艳）; (唐正午）; (赵维超）; (丁德馨）;

机构地区： 南华大学药学与生物科学学院,湖南衡阳421001

出　　处： 《湖南生态科学学报》 2017年第2期31-35,共5页

摘　　要： 探讨不同剂量的Cs^(137)对甲状腺FRTL细胞RET蛋白以及下游MAPK信号通路的影响.通过不同剂量(0.05,0.1,0.15,0.2,0.5,1 Gy)Cs^(137),对甲状腺FRTL细胞进行辐照,MTT法检测细胞增殖抑制率,Western blot检测未辐照组,辐照敏感组和辐照抗性组中RET及下游RAS、RAF、MEK蛋白表达情况.结果:甲状腺FRTL细胞在0.2Gy辐照时辐照敏感度增加;而到1 Gy辐照时辐照抗性增加.在0.2 Gy辐照敏感条件下,诱导RET、RAS、RAF、MEK蛋白表达下调;而当1 Gy辐照时,RET、RAS、BRAF、MEK蛋白表达上调.不同剂量Cs^(137)辐照能够诱导RET蛋白表达,对后面MAPK信号通路存在影响,最终影响到甲状腺FRTL细胞的细胞增殖抑制率,诱导甲状腺低剂量辐射致癌成为可能. Objective： To investigate the effect of different doses of Cs137 on RET protein and MAPK signaling pathway in FRTL cells. Methods ： Thyroid FRTL cells were irradiated with different doses （ 0.05,0.1,0.15,0.2,0,5,1 Gy） Cs137. MTT assay was used to detect the cell proliferation inhibition rate. The expression of RET, RAS, RAF and MEK protein in untreated group, irradiated Sensitive group and irradiated resistant group were detected by Western blot. Results： The irradiation sensitivity of thyroid FRTL cells decreased at 0.2 Gy, while the irradiation resistance increased when irradiated at 1 Gy. The expression of RET, RAS, RAF and MEK protein was down-regulated under 0.2 Gy irradiation, while the expression of RET, RAS, RAF and MEK was up-regulated when 1 Gy was irradiated. Conclusion： Different doses of Cs137 can induce the expression of RET protein,which may affect the subsequent MAPK signal pathway, and ultimately affect the cell proliferation inhibition rate of FRTL ceils and induce the thy- roid cancer.3figs., 2tabs., 12refs.

关 键 词： 甲状腺 细胞 低剂量辐射 信号