作　　者： (赵博）; (田恒康）; (孙道涵）; (申立峰）; (孔静）; (路东波）; (李文霞）; (武秋红）; (刘璐）; (李倩）; (张元元）; (吕珊）; (陈梦杰）; (石任兵）;

机构地区： 北京中医药大学中药学院国家中医药管理局中药经典名方有效物质发现重点实验室,北京100102

出　　处： 《北京中医药大学学报》 2017年第8期684-692,共9页

摘　　要： 目的建立基于药物体系的山楂特征图谱质量表征关联分析方法与评价模式,有效、精准地评价山楂饮片质量。方法运用所建立的HPLC-PDA法表征山楂药物体系特征图谱质量,基于特征图谱中特征峰的数量及所属化学类型对11批山楂饮片进行质的表征,基于特征图谱中特征指标性成分金丝桃苷、表儿茶素、原儿茶酸和绿原酸的含量及黄酮类(以金丝桃苷、表儿茶素表征)、酚酸类(以原儿茶酸和绿原酸表征)的含量对11批山楂饮片进行量的表征,并基于基准饮片将11批山楂饮片质与量的表征结果分别进行关联性分析。结果以批号1的样品为基准饮片,山楂饮片特征图谱中共含有特征峰13个,其中黄酮类成分特征峰7个,酚酸类特征峰6个,11批饮片色谱图中均含有此13个特征峰。批号5、10、8、2、6样品特征指标性成分含量总体较高,批号6、4、3、7、8、2样品与基准饮片关联性最高,综合评价得出批号5、10、8、2、7、3样品良度居前。结论对11批山楂饮片进行特征图谱质量表征,所建立的HPLC法简便、准确。所评价建立的基于药物体系的山楂特征图谱质量表征关联分析模式,关注了山楂饮片质量整体关联性与应用有效性,能有效、精准地评价山楂饮片质量。 Objective To establish quality representation and correlation analysis method based on the spectrum of medicinal system of Shanzha （ Fructus Crataegi, Chinese hawthorn fruit）, so as to evaluate quality of Shanzha decocting pieces effectively and accurately. Methods HPLC-PDA method was ap- plied to characterize quality of spectrum of Shanzha medicinal system; quality of 11 batches of Shanzha decocting pieces were characterized based on the number and chemical type of peaks on characteristic spectrum. The content of characteristic index components, protocatechuic acid, epicatechin, chlorogeni-cacid, phenolic acids （represented by protocatechuic acid and chlorogenic acid） and flavonoids （repre- sented by hyperoside and epicatechin） were characterized by indexes in characteristic spectrum. Correla- tion analysis of quality and quantity of Shanzha was then performed with reference to the standard refer- ence pieces. Results As the reference substance, characteristic spectrum of batch 1 contains 13 charac- teristic peaks, 7 peaks of flavonoids and 6 peaks of phenolic acids. All characteristic peaks appeared on the chromatograms of 11 batches of Shanzha. Batch 5,10, 8, 2,6 had more effective index ingredients. After comprehensive evaluation,there was a high correlation between batch 6, 4, 3, 7, 8, 2 and standard reference pieces. The quality of batch 5, 10, 8, 2, 7, and 3 ranked on the top among all samples. Con- clusion HPLC method to characterize quality and quantity of characteristic spectrum of 11 batches of Shanzha is simple and accurate. The evaluation mode of quantity and quality and their correlation based on Shanzha medical system, including systemic correlation and application validity, can evaluate the quality of hawthorn pieces effectively and accurately.

关 键 词： 山楂 特征图谱 质量评价 关联分析