作　　者： (郭玉萍）; (李恩中）; (张由婧）; (王安利）;

机构地区： 北京体育大学运动康复系,北京100084 安阳师范学院体育学院,河南安阳455000

出　　处： 《中华男科学杂志》 2017年第9期776-781,共6页

摘　　要： 目的:探讨有氧运动对雄性大鼠生精功能的影响,并通过蛋白组学筛选睾丸中有生精调控作用的差异表达蛋白,为进一步研究其分子机制奠定基础。方法:24只SD雄性大鼠随机分为对照组和运动组,对照组不进行运动训练。运动组分为无负荷运动组和3%体重负荷运动组,每天连续游泳60 min,无负荷运动组为无负重游泳,3%体重负荷运动组大鼠负荷3%体重重物游泳,每周6次,运动共9周。大鼠末次运动24 h后进行精子计数、生殖激素测定和睾丸组织学分析,并将3%体重负荷运动组和对照组睾丸组织进行同重同位素相对与绝对定量(iTRAQ)蛋白组学分析。结果:3%体重负荷运动组大鼠精子浓度[(3.54±0.52)×10~6/ml]明显高于对照组[(2.12±0.43)×10~6/ml],P<0.01;血清LH[(38.96±1.34)IU/L]和T[(21.36±0.53)nmol/L]也显著高于对照组[LH:(35.99±2.90)IU/L,T:(19.99±0.25)nmol/L,P均<0.01];GnRH和FSH两组无显著差异[GnRH:(641.82±42.78)ng/L vs(623.95±41.44)ng/L,FSH:(22.29±2.31)IU/L vs(20.49±2.44)IU/ml,P均>0.05]。无负荷运动组精子浓度、生殖激素水平与对照组相比均无显著变化。组织学分析显示3%体重负荷运动组大鼠生精上皮内细胞层数较对照组明显增多,成熟精子数增加。蛋白组学分析筛查到差异蛋白47个,其中上调蛋白37个,下调蛋白10个。经过生物信息学分析筛选出与生殖功能密切相关的显著差异表达蛋白5个,其中Anx A1、GPX3、Rimbp3、Dpy19l2表达上调,CYP17表达下调。KEGG通路富集分析显示,差异蛋白主要参与了细胞外基质-受体相互作用通路、蛋白质消化与吸收通路、黏着斑信号通路等。结论:有氧运动可能主要通过改变精子发生与分化相关的蛋白表达来提高机体的生精功能。 Objective： To investigate the effect of aerobic exercise on the spenatogenic function of male rats and screen out differentially expressed proteins related to spermatonesis-regulation by proteomic analysis. Methods： We randomly divided 24 SD male rats into groups A （non-exercise control）, B （exercise）, and C （weight-bearing exercise）, those in the latter two groups made to swim for 60 minutes a day and those in group C bearing a load 3% of the body weight, both 6 times a week for 9 weeks. At 24 hours after the last exercise, we obtained the sperm count, measured the levels of such serum reproductive hormones as testosterone （T）, luteotrophic hormone （ LH）, follicle-stimulating hormone （ FSH）, and gonadotrophin-releasing hormone （ GnRH）, and employed iso- baric tags for relative and absolute quantitation （iTRAQ） -based proteomic analysis of the testicular tissue. Results ： Compared with group A, group C showed significant increases in sperm concentration （ [ 2.12 ± 0.43 ] vs [ 3.54 ± 0.52 ] x 106/ml, P 〈 O. 01 ） and the levels of serumLH （[35.99± 2.90] vs [38.96 ± 1.34] IU/L,P 〈0.01） andT（[19.99 ± 0.25] vs [21.36 ＋ 0.53] nmol/L, P 〈 0.01 ）, but no statistically significant differences in GnRH （[ 623.95 ±41.44 ] vs [ 641.82 ±42.78 ] ng/L, P 〉 O. 05） and FSH （ [ 20.49 ±2.44 ] vs [ 22.29 ± 2.31 ] IU/L, P 〉 0.05）. No significant changes were observed in sperm concen- tration or reproductive hormone levels in group B as compared with A. Group B exhibited obviously more mature sperm and cell layers in the seminiferous epithelium than group A. A total of 47 differentially expressed proteins were identified, of which 37 were up-regula- ted and the other 10 down-regulated. In addition, another 5 significantly differentially expressed proteins closely related to reproductive function were identified, including up-regulated Anx A1, GPX3, Rimbp3, and Dpy1912 and down-regulated CYP17. Enrichment anal- ysis showed that the differentially expressed pr

关 键 词： 有氧运动 生精机能 同重同位素相对与绝对定量 蛋白组学 大鼠