作　　者： (陈兴隆）; (董明）;

机构地区： 天津医科大学,天津300070

出　　处： 《临床肺科杂志》 2017年第10期1863-1865,共3页

摘　　要： 目的探讨肺磨玻璃结节患者血浆中miR-10b作为诊断标记物的可能性。方法收集48例肺磨玻璃结节患者血浆,经术后病理证实良性病变16例,癌前病变(AAH,AIS)16例,微浸润性腺癌(MIA)8例,浸润性腺癌(IA)8例,采用实时荧光定量PCR检测各组血浆miRNA-10b水平,分析miRNA-10b与肺磨玻璃结节不同病理类型之间的关系。绘制ROC曲线,评估其诊断意义。结果肺癌相关组血浆miR-10b水平(RQ=0.828(0.485,1.700))较良性病变组(RQ=0.458(0.271,0.698))上调1.808倍,差异具有统计学意义(P=0.007);血浆miR-10b RQ值为0.731时,诊断微浸润腺癌(MIA)的AUC为0.827,敏感性为100%,特异性为67.5%;RQ值为1.137时,诊断浸润性腺癌(IA)的AUC为0.853,敏感性为75%,特异性为87.5%。结论GGO结节患者肺癌相关病变血浆miR-10b显著上调,特别是对微浸润腺癌和浸润性腺癌有一定诊断意义,有望成为GGO结节诊断标记物。 Objective To investigate the possibility of the expression of miR-lOb as the diagnostic marker in patients with ground-glass opacity nodules. Methods The expression of miR-lOb was detected by real-time quantita-tive PCR in 48 patients with ground-glass opacity nodules. The relationship between miR-lOb expression and clinical characteristics was analyzed. ROC was drawn to evaluate the diagnostic value of miR-lOb to the diagnosis of lung ade-nocarcinoma. Results The expression of plasma miR-lOb of the lung relation group [ RQ = 0. 828 （ 0. 485, 1. 700） ] was 1. 808 times the control group [ RQ =0. 458 （0. 271, 0. 698） ] （P = 0. 007）. while the expression of miR-lOb was 0.731, the diagnosis MIA sensitivity was 100%, the specificity was 82.4% and AUC was 0.827. While the expression was 1. 137, the diagnosis IA sensitivity was 75%,the specificity was 82. 4% and AUC was 0. 853. Conclusion The plasma miR-lOb level is up-regulated in patients with lung cancer related patients, which suggests miR-lOb might be used as a potential tumor marker for the diagnosis of ground-glass opacity nodules.

关 键 词： 肺磨玻璃结节 诊断标记物