作　　者： (张莹）; (楚启玉）; (陈淼）; (陈军）; (肖明）; (张永明）;

机构地区： 上海师范大学生命与环境科学学院,上海200234

出　　处： 《上海师范大学学报（自然科学版）》 2017年第4期453-459,共7页

摘　　要： 氯过氧化物酶(CPO)催化苯酚与H_2O_2发生过氧化反应生成邻苯二酚,能减轻苯酚对降解菌株的抑制作用,加快降解菌株对苯酚的生物降解.实验结果表明:在2 h内适量的H2O2存在时10 U/L的CPO可以使300 mg/L苯酚降解率达到67.85%,而CPO与降解菌株协同作用下苯酚降解率则可达到70.72%,比单一菌株降解率8.52%提高了62.2%.在降解体系中补充邻苯二酚进一步揭示了CPO氧化苯酚的中间产物有利于菌体细胞形成共基质效应,提高细胞的苯酚生物降解效率.降解动力学分析显示:在苯酚质量浓度为100~1 200 mg/L时,CPO与菌株协同降解体系的最大比降解速率qmax=0.000195 h-1,基质饱和常数Ks=1.0501 mg/L,基质抑制常数KI=5.1272 mg/L. Chloroperoxidase （CPO） can catalyze phenol reacting peroxide reaction generating with H2O2 to catechol,which can re-duce the inhibitory effect of phenol degradating generating bacterial strains. At the meantime,it can accelerate the rate of phenols biodegradation. The results show that 10 U/L of CPO has 67. 85% conversion rate of 300 mg /L phenol within 2 h with an appropri-ate amount of H2O2. While the degradation rate of phenol degradation under synergy of strain and CPO is up to 70. 72% increased by 62.2% comparing with a single strain degradation rate （8.52% ） . Supplementary catechol in the reaction system can further ver-ified that the intermediate products can be good for forming the co-substrate effect in bacteria, thereby improving the phenol degrada-tion efficiency of biological bacterial cells. Biodegradation dynamics analysis shows that the maximum specific degradation rate of the CPO and strain synergistic. The maximum specific degradation rate qmax = 0. 000195 h 1 , the matrix saturation constant Ks = 1.0501 mg/L,and the substrate inhibition constant Kj = 5. 1272 mg/L when phenol concentration in the range of 100 - 1 200 mg/L.

关 键 词： 氯过氧化物酶 过氧化作用 协同生物降解 苯酚残留 降解动力学