作　　者： (丁小丽）; (夏宇）; (汪琪）; (许培华）; (甘钰）; (李涛）; (黄帝锡）; (胡蓉）;

机构地区： 赣南医学院2014级研究生,江西赣州341000

出　　处： 《赣南医学院学报》 2017年第4期509-512,515,共5页

摘　　要： 目的:寻找有效、易检测的标志物并联合运用,为诊断急性肾损伤提供理论依据。方法:将SD大鼠随机分为假手术组和缺血再灌注组,夹闭双侧肾蒂阻断血流45 min构建RIRI模型。再灌注24 h后观察肾脏病理改变,检测血清CRE、BUN、SOD、MDA、hs-CRP及GSH-PX水平评价肾功能和氧化应激程度。结果:与Sham组相比,再灌注24 h后RIRI组血清肾功能指标CRE、BUN及肾脏病理损害评分较对照组均显著升高(P<0.001),HE染色结果显示肾小管及间质出现明显病理改变;同时RIRI组中大鼠血清SOD及GSH-PX活性显著降低,而hs-CRP、MDA水平则明显升高。结论:联合检测SOD、GSH-PX、hs-CRP及MDA水平可帮助诊断大鼠肾缺血再灌注引起的急性肾损害。 Objective: To find an effective and easily detectable marker in order to provide a theoretical basis for the diagnosis of acute renal injury. Methods: SD rats were randomly divided into Sham group and ischemia-reperfusion group. The bilateral renal pedicle was occluded to block the blood flow for 45 min to construct the RIRI model. The levels of CRE,BUN,SOD,MDA,hs-CRP and GSH-PX were measured after 24 hours reperfusion to evalue the renal function and degree of oxidative stress. Results: Compared with Sham group,the scores of CRE,BUN and renal pathological damage of RIRI group were significantly higher than those of control group( P < 0. 001); HE staining showed there was obvious pathological changes in renal tubular and interstitium. In the meantime,the activities of serum SOD and GSH-PX in RIRI group were clearly decreased,while the levels of hs-CRP and MDA were significantly increased. Conclusion: Combined detection of SOD,GSH-PX,hs-CRP and MDA levels may contribute to diagnose acute renal damage which caused by renal ischemia-reperfusion in rats.

关 键 词： 缺血再灌注 超氧化物歧化酶 谷胱甘肽过氧化物酶 超敏 反应蛋白 丙二醛 急性肾损伤