作　　者： (彭小进）; (王天淼）; (孙墨珑）;

机构地区： 东北林业大学理学院,黑龙江哈尔滨150040

出　　处： 《福建农业学报》 2017年第7期746-750,共5页

摘　　要： 采用核桃楸叶为原料,超声波辅助提取和紫外分光光度计对其多酚类物质进行含量测定。在单因素试验基础上,通过响应面法优化了核桃楸叶多酚类物质的提取工艺,得出核桃楸叶多酚类物质最佳提取条件为:乙醇体积分数70%,液料比15∶1,超声时间30min,超声温度60℃。核桃楸叶多酚类物质对羟基自由基、亚硝酸根离子、超氧阴离子、DPPH自由基有较强的清除能力,其对4种自由基的清除能力强弱顺序为DPPH自由基>超氧阴离子>羟基自由基>亚硝酸根离子,核桃楸叶多酚类物质对4种自由基的清除能力整体强于化学防腐剂苯甲酸,这表明核桃楸叶多酚类物质具有较强的抗氧化活性,是一种天然的抗氧化剂,为下一步研究多酚类物质的抗氧化性提供了依据。 Polyphenols were extracted from the leaves of Juglans mandshurica Maxim using an ultrasound-assisted process.Polyphenol content was determined by UV spectrophotometry.The extraction was optimized by a response surface experiment to arrive at the conditions including 70% by volume of ethanol as solvent,a solvent:solid ratio of 15:1,and an ultrasonic treatment of 30 min at 60℃.The polyphenol extract showed an excellent capacity in scavenging free radicals and ions in the order of DPPH radical>superoxide anion> hydroxyl radical> nitrite ion.It was more powerful than what the chemical preservative,such as benzoic acid,can provide.Thus,the polyphenols extracted from leaves of J.mandshurica by the current method could be a new natural antioxidant for potential applications.

关 键 词： 核桃楸叶 响应面法 多酚 抗氧化